Anti-Human IgM (μ-chain specific)−Peroxidase antibody produced in goat
SIGMA/A6907 - affinity isolated antibody, buffered aqueous solution
Synonym: Goat Anti-Human IgM (μ-chain specific)−HRP
MDL Number: MFCD00162464
Product Type: Chemical
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| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| concentration | 0.5-4 mg/mL |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| isotype | IgM |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:10,000 |
| Application: | Anti-Human IgM (μ-chain specific)-Peroxidase antibody has been used in direct binding assays to detect antiphospholipid antibodies (aPL). |
| Application: | Anti-human IgM (μ-chain specific)-Peroxidase antibody is suitable for use in direct ELISA (1:10,000 and 1:5000). It has also been used in indirect ELISA (1:2000). The concentration of IgM in supernatant samples was determined by ELISA using HRP-conjugated goat anti-human chain specific IgG as the secondary antibody. The antibody has also been used in Western blotting. |
| Biochem/physiol Actions: | Immunoglobulin M (IgM) acts as an antigen specific part of the B cell antigen receptor on the surface of B lymphocytes that are not stimulated, in its monomeric form. Polymeric IgM molecules also serve as important activators of the classical complement cascade. IgM is essential in agglutination and cytolytic reactions. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Human IgM antibodies are glycoproteins that are mainly produced by the B-1 cells and regulate the physiology and growth of the human B cell reserve. Circulating monoclonal IgM antibody that binds to myelin-associated glycoprotein has been linked to the demyelination of peripheral nerves in humans . Anti-human IgM μ-chain specific−peroxidase antibody is specific for human IgM when tested against human IgA, IgG, IgM, Bence Jones κ and Lambda myeloma proteins. |
| General description: | There are five immunoglobulin classes in humans. Out of these, immunoglobulin M (IgM) is a high molecular weight protein that has five to six subunits. IgM monomers are made of two heavy chains and two light chains connected by a disulfide bond. Human serum shows low concentration of IgM monomers. It has a high carbohydrate content of about 12%. It is the first immunoglobulin produced by neonates. |
| Immunogen: | human IgM |
| Packaging: | 1 mL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% BSA with preservative. |
| Preparation Note: | Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978). |
| Symbol |   GHS07,GHS09 |
| Signal word | Warning |
| Hazard statements | H315 - H317 - H319 - H411 |
| Precautionary statements | P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |