Alcohol Oxidase from Candida boidinii
SIGMA/A6941 - lyophilized powder, 5-15 units/mg protein
Synonym: AOD1; AOX; Alcohol:oxygen oxidoreductase
CAS Number: 9073-63-6
EC Number: 232-971-3
MDL Number: MFCD00130452
Product Type: Chemical
Unit Definition One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25 °C.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A6941-250UN
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A6941-50UN
| biological source | fungus (Candida boidinii) |
| form | lyophilized powder |
| mol wt | octomer 600 kDa by sedimentation equilibrium |
| Quality Level | 200  |
| solubility | 100 mM potassium phosphate, pH 7.5: soluble 1.0 mg/mL at 25 °C (Cold) |
| specific activity | 5-15 units/mg protein |
| storage temp. | −20°C |
| Application: | Alcohol oxidase has been used: • to catalyze the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes . • to study methanol metabolism in yeasts, such as Candida, Pichia, and Hansenula. • to study protein translocation into peroxisomes. • for the determination of ethanol concentration in alcoholic drinks using enzymatic assay. • in development of enzyme electrode for the determination of alcohols. |
| Biochem/physiol Actions: | Alcohol oxidase has the highest affinity for methanol. The affinity decreases with increasing chain length of the alkyl (R) group. The enzyme shows little activity toward secondary, tertiary, or aromatic alcohols; or aliphatic alcohols with a chain length of more than 5 carbons. The pH range for activity of this product is 6.5-8.5, with the optimum pH being 7.5. Alcohol oxidases facilitate the conversion of alcohol into carbonyl compounds while producing hydrogen peroxide. It is also the first enzyme in the yeast methanol utilization pathway. |
| General description: | Research area: Cell Signaling Alcohol Oxidase (AOX) is a homo-octamer composed of eight flavin adenine dinucleotide (FAD) cofactors and belongs to the glucose-methanol-choline (GMC) family of oxidoreductases. The AOX1 and AOX2 genes are responsible for encoding AOX. Alcohol oxidase is primarily localized in the peroxisome but is also found in the cytoplasm. Alcohol oxidase is a 600 kDa homooctomeric flavoprotein with eight equal 74 kDa subunits; each containing a flavin adenine dinucleotide (FAD) molecule. |
| Other Notes: | One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25 °C. |
| Packaging: | 50, 250 units in glass bottle |
| Physical form: | Contains potassium phosphate buffer salts, DTE, and stabilizer |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 5-15 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.1.3.13 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |