Anti-AtMPK4 antibody produced in rabbit
SIGMA/A6979 - affinity isolated antibody, liquid
Synonym: Anti-Arabidopsis thaliana MPK4
MDL Number: MFCD07370260
Product Type: Chemical
Immunoblotting Arabidopsis thaliana whole leaf extracts (Lanes A, F), seedlings (Lanes B, E), flowers (Lane C) and roots (Lane D) were separated on SDS-PAGE and blotted with Rabbit Anti-AtMPK4 (Cat. No. A6979). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody concentration 1.0 μg/mL Specificity is demonstrated by inhibition of antibody staining of whole leaf extract with the AtMPK4 immunizing peptide (20 μg/mL, Lane F).
Immunoblotting
Arabidopsis thaliana whole leaf extract was separated by SDS-PAGE and blotted with: (Lane A) Rabbit Anti-AtMPK3 (0.5 μg/mL) (Cat. No. M8318); (Lane B) Rabbit Anti-AtMPK4 (0.5 μg/mL), (Cat. No. A6979); (Lane C) Rabbit Anti-AtMPK6 (0.25 μg/mL), (Cat. No. A7104). The antibodies were developed with Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Arabidopsis thaliana whole leaf extract was separated by SDS-PAGE and blotted with: (Lane A) Rabbit Anti-AtMPK3 (0.5 μg/mL) (Cat. No. M8318); (Lane B) Rabbit Anti-AtMPK4 (0.5 μg/mL), (Cat. No. A6979); (Lane C) Rabbit Anti-AtMPK6 (0.25 μg/mL), (Cat. No. A7104). The antibodies were developed with Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | liquid |
| grade | Molecular Biology |
| mol wt | antigen 43 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | Arabidopsis thaliana |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.5-1 μg/mL using extract (cytosolic fraction) of Arabidopsis thaliana and Nicotiana tabacum leaves |
| Application: | Anti- mitogen-activated protein kinase-4 (AtMPK4) antibody produced in rabbit has been used for Western blotting. |
| Application: | Anti-AtMPK4 antibody has been used in • immunoblotting • immunoprecipitation • co-immunoprecipitation • western blotting |
| Biochem/physiol Actions: | In Arabidopsis thaliana putative functions have been assigned to AtMPK3, AtMPK4, and AtMPK6, and all three mitogen-activated protein kinase (MAPKs) have been linked to diverse stress responses, including oxidative stress signaling, pathogen defense, drought, high salt, cold, and touch. Arabidopsis MAPKs, Anti- mitogen-activated protein kinase-3 (AtMPK3), Anti- mitogen-activated protein kinase-4 (AtMPK4), and Anti- mitogen-activated protein kinase-6 (AtMPK6) are rapidly and transiently activated by stress responses, by activation of distinct signal transduction pathways, involving upstream MAPKKKs (e.g. AtANP1 and AtMEKK1) and MAPKKs including AtMKK1/2 and AtMKK4/5. |
| Biochem/physiol Actions: | Mitogen-activated protein kinase-4 (MPK4) negatively modulates systemic-acquired resistance. It functions as a regulator of retrograde signaling for photosynthesis, growth and immune defenses. It also has a role in cytokinesis and microtubule organization. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-AtMPK4 is produced in rabbit using as immunogen a synthetic peptide corresponding to the C-terminus of Arabidopsis thaliana MPK4 (Anti- mitogen-activated protein kinase-4), conjugated to KLH. In Arabidopsis thaliana, 20 genes (MPKs) encode putative plant MAPKs, which can be grouped into four or five classes according to sequence similarity. All plant mitogen-activated protein kinase (MAPKs) are classified into the plant extracellular-regulated kinase (PERK) subfamily. |
| General description: | Mitogen-activated protein kinase-4 (MPK4) is expressed in the nucleus. In pre-mitotic and interphase cells, it is found in the cytoplasm. |
| Immunogen: | synthetic peptide corresponding to the C-terminus of Arabidopsis thaliana MPK4 (amino acids 365-376). This sequence is specific to AtMPK4 (not found in the other 20 known AtMPK isoforms). It shares limited homology (50-60% identity, continuous epitope) to the AtMPK5 and AtMPK12 isoforms. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |