β-Amylase from sweet potato
SIGMA/A7005 - Type I-B, ammonium sulfate suspension, ≥750 units/mg protein (E1%/280 )
Synonym: 1,4-α-D-Glucan maltohydrolase
CAS Number: 9000-91-3
EC Number: 232-566-1
MDL Number: MFCD00081391
Product Type: Chemical
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 5—Safer Solvents and Auxiliaries. This product utilizes reduced volumes of auxiliary substances that are less hazardous.
| biological source | sweet potato |
| concentration | ≥7.0 mg/mL |
| form | ammonium sulfate suspension |
| greener alternative category |  , Re-engineered |
| greener alternative product characteristics | Waste Prevention Safer Solvents and Auxiliaries Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| greener alternative product score | old score: 57 new score: 1 Find out more about DOZN™ Scoring |
| Quality Level | 200 |
| specific activity | ≥750 units/mg protein (E |
| storage temp. | 2-8°C |
| sustainability | Greener Alternative Product |
| type | Type I-B |
| Application: | β -Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. β -Amylase, from Sigma, has been used in various plant studies, such as carbon starvation studies in Populus tremuloides. β-amylase from sweet potato has been used to examine the utility of the enzyme in inhibiting and removing Staphylococcus aureus biofilms. The enzyme has also been used to prepare β-limit dextrin from waxy maize starch. |
| Application: | β-Amylase from sweet potato has been used: • in the hydrolysis of starch and starch-pectin combinations • for comparing amylases from different sources in Staphylococcus aureus SH1000 biofilm inhibition and reduction assay • for selenium speciation in samples |
| Biochem/physiol Actions: | β-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. β-amylase from sweet potato is a tetramer of identical subunits, which are arranged to exhibit 222 molecular symmetry. |
| Biochem/physiol Actions: | β-Amylases plays a major role in examining enzymatic promiscuity among glycosidases. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one of the four categories of Greener Alternatives . This product belongs to category of Re-engineered products, showing key improvements in Green Chemistry Principles “Waste Prevention”, “Safer Solvents and Auxiliaries” and “Enhanced Energy Efficiency”. Click here to view its DOZN scorecard. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles . |
| General description: | β-Amylases belongs to the class of amylolytic enzymes. |
| Other Notes: | One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 4.8 at 20 °C. |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Packaging: | 10000, 25000, 50000, 100000 units in glass bottle |
| Physical form: | Crystalline suspension in 2.3 M (NH4)2SO4 |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P342 + P311 |
| Hazard Codes | Xn |
| Risk Statements | 42 |
| Safety Statements | 22-24-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| activity | specific activity: ≥750 units/mg protein (E |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.2 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |