Anti-AtMPK6 antibody produced in rabbit
SIGMA/A7104 - affinity isolated antibody, liquid
Synonym: Anti-Arabidopsis thaliana MPK6
MDL Number: MFCD07370261
Product Type: Chemical
Immunoblotting Arabidopsis thaliana whole seedlings (Lane A), leaf extracts (Lanes B, E), flowers (Lane C) and roots (Lane D) were separated on SDS-PAGE and blotted with Rabbit Anti-AtMPK6 (Cat. No. A7104). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545 and chemiluminescent substrate. Antibody concentration 1.0 μg/mL. Specificity is demonstrated by inhibition of antibody staining of whole leaf extract with the AtMPK6 immunizing peptide (20 μg/mL, Lane E).
Immunoblotting
Arabidopsis thaliana whole leaf extract was separated by SDS-PAGE and blotted with: (Lane A) Rabbit Anti-AtMPK3 (0.5 μg/mL) (Cat. No. M8318); (Lane B) Rabbit Anti-AtMPK4 (0.5 μg/mL), (Cat. No. A6979); (Lane C) Rabbit Anti-AtMPK6 (0.25 μg/mL), (Cat. No. A7104). The antibodies were developed with Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Arabidopsis thaliana whole leaf extract was separated by SDS-PAGE and blotted with: (Lane A) Rabbit Anti-AtMPK3 (0.5 μg/mL) (Cat. No. M8318); (Lane B) Rabbit Anti-AtMPK4 (0.5 μg/mL), (Cat. No. A6979); (Lane C) Rabbit Anti-AtMPK6 (0.25 μg/mL), (Cat. No. A7104). The antibodies were developed with Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | liquid |
| grade | Molecular Biology |
| mol wt | antigen 47 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | Arabidopsis thaliana |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.25-0.5 μg/mL using extract (cytosolic fraction) of Arabidopsis thaliana leaves |
| Application: | Anti-AtMPK6 antibody produced in rabbit has been used in immunoblotting. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | Mitogen-activated protein kinase (MAPK) cascades are among the most conserved signal transduction pathways in eukaryotes. MAPKs regulate a variety of cellular activities ranging from gene expression, cell proliferation, motility, metabolism and apoptosis. In Arabidopsis thaliana putative functions have been assigned to AtMPK3, AtMPK4 and AtMPK6 and all three MAPKs have been linked to diverse stress responses, including oxidative stress signaling, pathogen defense, drought, high salt, cold and touch. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | In Arabidopsis thaliana, 20 genes (MPKs) encode putative plant MAPKs, which can be grouped into four or five classes according to sequence similarity. All plant MAPKs are classified into the plant extracellular-regulated kinase/ERK (PERK) subfamily. |
| Immunogen: | synthetic peptide corresponding to the C-terminus of Arabidopsis thaliana MPK6 (amino acids 384-395). This sequence is specific to AtMPK6 (not found in the other 20 known AtMPK isoforms). It shares limited homology (40-50% identity) to the AtMPK3 and AtMPK4 isoforms, and is highly conserved in the Nicotiana tabacum ortholog SIPK. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |