β-Amylase from barley
SIGMA/A7130 - Type II-B, 20-80 units/mg protein (biuret)
Synonym: 1,4-α-D-Glucan maltohydrolase
CAS Number: 9000-91-3
EC Number: 232-566-1
MDL Number: MFCD00081391
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A7130-250KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A7130-10KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A7130-50KU
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
| form | powder |
| greener alternative category |  , Enabling |
| greener alternative product characteristics | Waste Prevention Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| Quality Level | 200 |
| shipped in | wet ice |
| specific activity | 20-80 units/mg protein (biuret) |
| storage temp. | 2-8°C |
| sustainability | Greener Alternative Product |
| type | Type II-B |
| Analysis Note: | Crude |
| Application: | β -Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. β -Amylase, has been used in various plant studies, such as carbon starvation studies in Populus tremuloides . β -Amylase, from barley, has been used to study how pressure and temperature affect catalytic activity . |
| Biochem/physiol Actions: | β-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. Pure, crystalline β-amylase preparation consists of four isoenzymes with different isoelectric points. The enzyme polymerizes very rapidly through the sulfhydryl groups in the absence of reducing agents. p-Chloromercuribenzoate inhibits the polymerization and the enzymatic activity. The reducing agents mercaptoethanol or dithiothreitol can completely restore the activity. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles . |
| Other Notes: | One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 4.8 at 20 °C. |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Packaging: | 10000, 50000, 250000 units in poly bottle |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P342 + P311 |
| Hazard Codes | Xn |
| Risk Statements | 42 |
| Safety Statements | 22-24-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 20-80 units/mg protein (biuret) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.2 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |