L-Alanine Dehydrogenase from Bacillus subtilis
SIGMA/A7189 - ammonium sulfate suspension, ≥20 units/mg protein (Lowry)
Synonym: L-Alanine: NAD+ oxidoreductase (deaminating)
CAS Number: 9029-06-5
EC Number: 232-847-9
MDL Number: MFCD00131441
Product Type: Chemical
Unit Definition One unit will convert 1.0 μmole of L-alanine to pyruvate and NH3 per min at pH 10.0 at 25 °C. β-NAD = β-Nicotinamide Adenine Dinucleotide, Oxidized Form. β-NADH = β-Nicotinamide Adenine Dinucleotide, Reduced Form.
| biological source | Bacillus subtilis |
| form | ammonium sulfate suspension |
| Quality Level | 200  |
| specific activity | ≥20 units/mg protein (Lowry) |
| storage temp. | 2-8°C |
| Application: | |
| Application: | L-Alanine dehydrogenase converts L-alanine to pyruvate and ammonium. L-Alanine dehydrogenase from Bacillus subtilis may be used to study enzyme inactivation and protection . |
| Biochem/physiol Actions: | |
| Biochem/physiol Actions: | L-Alanine dehydrogenase is a stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the generation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate and NADH are released in that specific order. Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent metal ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L- or D-cysteine. |
| General description: | L-Alanine Dehydrogenase has a N-terminal substrate-binding domain and a C-terminal NAD-binding domain. |
| Other Notes: | One unit will convert 1.0 μmole of |
| Packaging: | 250, 500 units in glass bottle |
| Physical form: | Suspension in 2.4 M (NH4)2SO4 solution, pH 7.0 |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P304 + P340 + P312 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| activity | specific activity: ≥20 units/mg protein (Lowry) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.4.1.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |