Avidin–Alkaline Phosphatase
SIGMA/A7294 - buffered aqueous solution
Synonym: Avidin–AP
MDL Number: MFCD00130582
Product Type: Chemical
ELISA assay utilizing a biotinylated antibody and Avidin−Alkaline Phosphatase detection.
Western Blotting Whole extract of HeLa cells were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-β-Actin antibody produced in Mouse, Clone: AC-15 (Cat. No. A5441). The antibody was developed using 1:20,000 Anti-Mouse IgG (whole molecule)-Biotin antibody produced in Rabbit (Cat. No. B8520) followed by Avidin-Alkaline Phosphatase (Cat. No. A7294). Lanes 1. 1:300,000 antibody 2. Negative Control
| biological source | avidin from egg white |
| enzyme from bovine (calf) intestine | |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| technique(s) | direct ELISA: 1:70,000 |
| western blot: 1:150,000-1:300,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per lane |
| Application: | Avidin-Alkaline Phosphatase has been used for ELISA. The product can also be used for western blot at 1:150,000-1:300,000 dilutions. |
| Application: | Avidin-Alkaline Phosphatase has been used in enzyme-linked immunosorbent assay (ELISA). |
| Application: | Cytokine ELISA Assays were performed using a biotinylated anti-IL-2 antibody and alkaline phosphatase avidin. |
| Biochem/physiol Actions: | Avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities. Avidin is a fatty acid biosynthesis regulator. It participates in terminal cell differentiation by weakening the multiplication of cell without affecting the differentiation process. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Avidin is homotetrameric protein (66 kDa) obtained from egg whites and binds strongly to biotin. It has four identical subunits of 16,400 Daltons each. It is an attractive adaptor protein, that is resistant to denaturation. This glycoprotein is present in avian, reptilian and amphibian egg white. The product is affinity purified egg white avidin (acitivity 10-15 units/mg protein) conjugated to alkaline phosphatase using a 0.2% glutaraldehyde method. |
| General description: | Avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities. |
| Physical form: | Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide. |
| Preparation Note: | Affinity purified protein conjugated to alkaline phosphatase using 0.2% glutaraldehyde. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |