Anti-Atg16L antibody produced in rabbit
SIGMA/A7356 - fractionated antiserum, buffered aqueous solution
Synonym: Anti-APG16L; Anti-ATG16 autophagy related 16-like 1 (S. cerevisiae); Anti-ATG16L1
Product Type: Chemical
Immunoblotting Whole extract of A549 cells was separated on SDS-PAGE and probed with Rabbit Anti-ATG16L (Cat. No. A7356). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 1:500 antibody 2. 1:1,000 antibody 3. 1:1,000 antibody + ATG16L immunizing peptide (mouse, 2-15)
Immunoblotting Whole extract of human HEK-293T cells overexpressing mouse ATG16L was separated on SDS-PAGE and probed with Rabbit Anti-ATG16L (Cat. No. A7356). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 1:500 antibody 2. 1:1,000 antibody 3. 1:1,000 antibody + ATG16L immunizing peptide (mouse, 2-15)
| antibody form | fractionated antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~70 kDa (several isonform bands) |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:500-1:1,000 using whole extracts of human A549 cells, rat PC12 cells, and HEK-293T cells expressing mouse Atg16l |
| UniProt accession no. | Q676U5  |
| Application: | Anti-Atg16L antibody produced in rabbit has been used in western blotting. |
| Biochem/physiol Actions: | Atg16L is involved in LC3 lipidation during the formation of autphagosomes. Studies have reported that Atg16L may also function as a mammalian Rab33 effector. |
| Biochem/physiol Actions: | Autophagy-related 16-like 1 (ATG16L1) modulates host immune responses and is linked with several diseases, like cardiovascular disease (CVD). Atg16 multimeric complex plays an essential role in autophagy. Atg16L interacts with both Atg5 and additional Atg16L monomers. Together with Atg12-Atg5 conjugate, Atg16L associates with the autophagic isolation membrane for the duration of autophagosome formation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | ATG16L is a component of a large protein complex that is critical for autophagy. |
| General description: | Autophagy-related 16-like 1 (ATG16L1) is an autophagy gene, that is expressed in the colon, small intestine, intestinal epithelial cells, leukocytes and spleen. Atg16L is expressed in different isoform patterns depending on the tissue. |
| Immunogen: | synthetic peptide correspopnding to amino acids 2-15 of mouse Atg16l, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and human. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |