Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat
SIGMA/A7434 - affinity isolated antibody, buffered aqueous glycerol solution
MDL Number: MFCD00162626
Product Type: Chemical
Thoracic and abdominal segments of a Drosophila melanogaster embryo double labeled using monoclonal antibodies to engrailed (purple) and achaete (brown) proteins. Engrailed expression marks the posterior compartment of each segment and achaete expression labels five neuroblasts per hemisegment: three found midway between engrailed stripes and two found coincident with engrailed stripes. The cellular outlines of non-achaete positive neuroblasts are clearly visible interspersed among the achaete positive neuroblasts. Goat Anti-Mouse IgG-Alkaline Phosphatase conjugate (Cat. No. A7434) and BCIP/NBT (Cat. Nos. B8503/N6876) and DAB tablets (Cat. No. D5905) were used. From J. Skeath, Univ. of Wisc., Madison, WI]
Immunohistochemistry Formalin fixed, paraffin-embedded Human appendix tissue sections were stained with 1:400 Monoclonal Anti-Actin, α-Smooth Muscle, Clone: 1A4 (Cat. No. A2547) followed by 1:160 Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A7434).
Western Blotting Whole extract of HeLa cells were separated on SDS-PAGE and probed with 2 μg/mL Anti-β-Actin antibody, Mouse monoclonal, Clone: AC-15 (Cat. No. A1978). The antibody was developed using Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A7434). Lanes 1. 30,000 antibody 2. 60,000 antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous glycerol solution |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:40,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 | |
| western blot (chemiluminescent): 1:30,000 using β-actin in total cell extract of HiLa cells (5-10 μg per well) |
| Application: | Alkaline phosphatase-conjugated goat anti-mouse Fc specific antibody was used as a secondary antibody in ELISA assays at a dilution of 1:1000 in PBS/0.1% Tween and 1% BSA for 1.5 hours at 37°C. Antibody was developed using 4-nitrophenyl phosphate (Sigma) as a substrate for 30 minutes at 37°C. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The product does not react with mouse IgG, Fab fragment, human IgG, IgA, and IgM, or rat IgG. |
| Immunogen: | Purified mouse IgG, Fc fragment |
| Other Notes: | Antibody adsorbed with human IgG and rat serum proteins. |
| Physical form: | Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide. |
| Preparation Note: | Adsorbed to reduce background staining with human or rat samples. |
| Specificity: | Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The product does not react with mouse IgG, Fab fragment, human IgG, IgA, and IgM, or rat IgG. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |