Acetate Kinase from Escherichia coli
SIGMA/A7437 - lyophilized powder, ≥150 units/mg protein (biuret)
Synonym: ATP:Acetate phosphotransferase
CAS Number: 9027-42-3
MDL Number: MFCD00130330
Product Type: Chemical
| composition | Protein, 5.0-20.0% |
| foreign activity | adenosine triphosphatase and NADH oxidase ≤0.01% |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | ≥150 units/mg protein (biuret) |
| storage temp. | −20°C |
| UniProt accession no. | P0A6A3  |
| Application: | Acetate Kinase from Escherichia coli has been used in assessing acetate kinase activity during the fermatation process of Gluconacetobacter xylinus and in kinetic modeling and steady state studies. |
| Application: | Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Escherichia coli has been used as part of an ATP-regenerating system to study the kinetics of agonist-stimulated transphosphatidylation . |
| Biochem/physiol Actions: | Acetate kinase plays an important role in glycolysis. Acetate kinase phosphorylates acetate in the presence of ATP and a divalent cation, which ultimately results in the production of acetyl-CoA . Acetate kinase is also involved in the metabolism of propanoate, pyruvate and taurine. |
| Biochem/physiol Actions: | Involved in the metabolism of propanoate, pyruvate and taurine |
| General description: | Acetate kinase belongs to acetate and sugar kinase/Hsc70/actin (ASKHA) structural superfamily. It exists as a dimer and has nucleotide binding site in the cleft between N and C-terminal domain. |
| Physical form: | Lyophilized powder containing trehalose with small amounts of potassium phosphate, magnesium chloride, and dithiothreitol |
| Unit Definition: | One unit will phosphorylate 1.0 μmole of acetate to acetyl phosphate per min at pH 7.6 at 25 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥150 units/mg protein (biuret) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.7.2.1 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |