Anti-c-Myc Agarose Affinity Gel antibody produced in rabbit
SIGMA/A7470 - affinity isolated antibody
Synonym: Anti-c-Myc
Product Type: Chemical
| analyte chemical class(es) | proteins (c-Myc) |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| capacity | ≥10 nmol/mL binding capacity (c-Myc tagged fusion protein) |
| ≥7 nmol/mL elution capacity (c-Myc tagged fusion protein) | |
| clone | polyclonal |
| conjugate | agarose conjugate |
| Quality Level | 200  |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | affinity chromatography: suitable |
| immunoprecipitation (IP): suitable | |
| UniProt accession no. | P01106  |
| Application: | Anti-c-Myc Agarose Affinity Gel antibody produced in rabbit has been used in: • chromatin immunoprecipitation (ChIP) • co-immunoprecipitation • western blotting • immunofluorescence • immunoprecipitation |
| Biochem/physiol Actions: | c-Myc proto-oncogene increased expression is observed in a variety of human tumors. Amplification of Myc gene locus is observed in angiosarcomas and translocation is implicated in Burkitt′s lymphoma. c-Myc is a master regulator and targets a wide variety of metabolic events. An epitope located within amino acid residues 410-419 (EQKLISEEDL) of human c-Myc has been widely used as a tag in many expression vectors, enabling the expression of proteins as c-Myc tagged fusion proteins. Anti-c-Myc agarose is useful in the purification of expressed c-Myc tagged fusion proteins in bacterial lysates or transfected cells. |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The human c-Myc proto-oncogene belongs to MYC oncogene family and is the human cellular homolog of the avian v-Myc gene found in several leukemogenic retroviruses. c-Myc gene is localized on human chromosome 8q24.21. The two major domains of include the N-terminal box regions and the C-terminal basic-region with helix-loop-helix/leucine- |
| Immunogen: | synthetic peptide of the human p62c-Myc protein. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Physical form: | Suspension of beaded agarose (1:1 v/v) in 0.01 M phosphate buffered saline containing 15 mM sodium azide. |
| Specificity: | Anti-c-Myc recognizes an epitope located on c-Myc tagged fusion proteins. |
| Storage and Stability: | For continuous use and extended storage, store at 2 °C to 8 °C. Do not freeze. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |