Anti-Atg1/ULK1 antibody produced in rabbit
SIGMA/A7481 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-UNC51; Anti-Unc-51 like kinase 1 (C. elegans); Anti-Unc51.1
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Western Blotting. Antibody recognition: Anti-ATG1/ULK1 antibody produced in Rabbit (Cat. No. A7481) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing ATG1/ULK1 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 0.5 μg/mL.
Immunoblotting Whole cell extract of Rat PC12 was separated on SDS-PAGE and probed with Rabbit Anti-ATG1/ULK1 (Cat. No. A7481). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody 2 μg/mL 2. Antibody 5 μg/mL 3. Antibody 5 μg/mL + ATG1/ULK1 immunizing peptide (mouse/rat, 415-430)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~150 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat, human (predicted), mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells expressing mouse ULK1 |
| western blot: 3-6 μg/mL using whole extracts of rat PC12 | |
| UniProt accession no. | O75385  |
| Application: | Anti-Atg1/ULK1 antibody produced in rabbit has been used in western blotting and immunohistochemical staining. |
| Biochem/physiol Actions: | Unc-51 like autophagy activating kinase 1 (ULK1) regulates autophagy initiation and innate immunity of interferons (IFNs). ULK1 is critical for neuronal development. It is essential for neurite elongation and differentiation of cerebellar granule neurons. Mutations in this gene is linked to latent mycobacterium tuberculosis infection (LTBI). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Atg1 is an autophagy protein that functions as a serine/threonine kinase in yeast to regulate autophagasome formation. Atg1 is ULK1 in mammals. |
| General description: | Unc-51 like autophagy activating kinase 1 (ULK1) gene is located on human chromosome 12q24.33. |
| Immunogen: | Synthetic peptide corresponding to amino acids of mouse Atg1/ULK1 conjugated to KLH via a cysteine residue. The corresponding sequence is identical in rat and differs by 3 amino acids in human. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Anti- Atg1/ULK1 recognizes rat and mouse Atg1/ULK1. |
| Storage and Stability: | For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |