Adenosine 5′-Triphosphatase from porcine cerebral cortex
SIGMA/A7510 - lyophilized powder, ≥0.3 units/mg protein, pH 7.8
Synonym: ATP phosphohydrolase; ATPase
CAS Number: 9000-83-3
MDL Number: MFCD00135671
Product Type: Chemical
| biological source | Porcine cerebral cortex |
| composition | Protein, 5.0-25.0% biuret |
| description | Sodium and Potassium-Activated |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | ≥0.3 units/mg protein, pH 7.8 |
| storage temp. | −20°C |
| technique(s) | inhibition assay: suitable |
| UniProt accession no. | P05024  |
| P05027 |
| Application: | ATPase is used to liberate inorganic phosphorus from ATP. ATPase, from porcine cerebral cortex, has been used in inhibition assays of Na/K-ATPase activity . |
| Biochem/physiol Actions: | This membrane enzyme hydrolyzes ATP to ADP and orthophosphate. The hydrolysis is coupled with the exchange of sodium and potassium ions across the plasma membrane. The enzyme is present in almost all tissues of higher organisms, but it is most abundant in the kidney where it reabsorbs sodium ions from the glomerular filtrate. Na+,K+-ATPase is affected by changes in intracellular calcium. It is also sensitive to ouabain and inhibited by vanadate, and (in vitro) by SPAI-1, a peptide isolated from porcine duodenum. It has been reported to be regulated by phosphorylation by protein kinase A (PKA). Bufalin, and other cardiotonic steroids, may inhibit Na+/K+-ATPase activity and induce the differentiation of HL60, U937, and ML1 cells . |
| Biochem/physiol Actions: | This membrane enzyme hydrolyzes ATP to ADP and orthophosphate. The hydrolysis is coupled with the exchange of sodium and potassium ions across the plasma membrane. The enzyme is present in almost all tissues of higher organisms, but it is most abundant in the kidney where it reabsorbs sodium ions from the glomerular filtrate. Na+,K+-ATPase is affected by changes in intracellular calcium. It is also sensitive to ouabain and inhibited by vanadate, and (in vitro) by SPAI-1, a peptide isolated from porcine duodenum. It has been reported to be regulated by phosphorylation by protein kinase A (PKA). |
| Physical form: | Lyophilized powder containing 90% sucrose, 0.4% EDTA Na4 and 0.06% NaCl |
| Preparation Note: | Sodium iodide extracted fraction of Nakao, et al., Biochem. Biophys. Res. Commun., 19, 755 (1965). |
| Unit Definition: | One unit will liberate 1.0 μmole of inorganic phosphorus from ATP per min at pH 7.8 at 37 °C in the presence of Na+, K+, and Mg2+. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥0.3 units/mg protein, pH 7.8 |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.6.1.3 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |