Monoclonal Anti-Bovine IgG−Alkaline Phosphatase antibody produced in mouse
SIGMA/A7554 - clone BG-18, purified immunoglobulin, buffered aqueous glycerol solution
Synonym: Monoclonal Anti-Bovine IgG
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded, Rabbit spleen tissue sections were stained with 1:50 Bovine Anti-Rabbit IgG followed by 1:150 Monoclonal Anti-Bovine IgG-Alkaline Phosphatase antibody produced in Mouse, Clone: BG-18 (Cat. No. A7554).
Western Blotting Rabbit IgG were separated on SDS-PAGE and probed with 1:1,000 Bovine anti-Rabbit IgG. The antibody was developed using Monoclonal Anti-Bovine IgG-Alkaline Phosphatase antibody produced in Mouse, Clone: BG-18 (Cat. No. A7554). Lanes 1. 1:100,000 antibody 2. 1:200,000 antibody 3. Negative Control
ELISA Monoclonal Anti-Bovine IgG-Alkaline Phosphatase antibody produced in Mouse, Clone: BG-18 (Cat. No. A7554) was serially diluted to detect Bovine IgG by direct ELISA. Reactivity is observed with Bovine IgG, Bovine IgG1 and Bovine IgG2 but not with Human IgG and Human IgM.
| antibody form | purified immunoglobulin |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | BG-18, monoclonal |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous glycerol solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | wet ice |
| should not react with | pig, human, rabbit, sheep, cat, guinea pig, canine, horse, goat |
| species reactivity | bovine (non-reactive to IgM) |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:25,000 |
| dot blot: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:75 using using rabbit spleen sections and bovine anti-rabbit IgG | |
| western blot (chemiluminescent): 1:100,000 |
| Application: | Monoclonal Anti-Bovine IgG?Alkaline Phosphatase antibody produced in mouse has been used in: • western blotting • enzyme linked immunosorbent assay (ELISA) • dot blot • immunohistochemistry |
| Biochem/physiol Actions: | Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Bovine IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| General description: | The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction. |
| Immunogen: | Purified bovine IgG |
| Physical form: | Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide |
| Specificity: | bovine IgG1 and IgG2 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |