α-Amylase from Bacillus amyloliquefaciens
SIGMA/A7595 - liquid, ≥250 units/g
Synonym: BAN™ 480L; 1,4-α-D-Glucan Glucano-hydrolase
CAS Number: 9000-85-5
EC Number: 232-560-9
MDL Number: MFCD00081319
Product Type: Chemical
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
| form | liquid |
| greener alternative category |  , Enabling |
| greener alternative product characteristics | Waste Prevention Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| mol wt | 55 kDa |
| Quality Level | 200 |
| specific activity | ≥250 units/g |
| storage temp. | 2-8°C |
| sustainability | Greener Alternative Product |
| Application: | α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. Product A7595 is from Bacillus amyloliquefaciens and is supplied as a liquid. α-Amylase has been used in various plant studies, such as metabolism studies in Arabidopsis. α-Amylase from Bacillus amyloliquefaciens has been used to hydrolyze sweetpotato amylopectin to identify cluster structure. The enzyme has been used to hydrolyze sago palm starch to reducing sugars, which are then used for ethanol fermentation by Saccharomyces cerevisiae FNCC 3012. The enzyme catalyzes amylolysis of gelatinised waxy maize starch to produce reducing sugars. |
| Biochem/physiol Actions: | α-Amylase is an endoamylase that randomly hydrolyzes the α-(1,4) glucan linkages in amylose and amylopectin. The breakdown products are oligosaccharides and dextrins of varying chain length. This enzyme works best at high temperatures, in the range of 70-90 ° Celcius. |
| Biochem/physiol Actions: | An endoamylase that randomly hydrolyzes α-(1→4)-glycosidic linkages in amylose and amylopectin. The breakdown products are oligosaccharides and dextrins of varying chain length. This enzyme is active at high temperatures (70−90 °C). |
| Biochem/physiol Actions: | The pH range for the α-amylase of B.amyloliquefaciens is 5.5 to 6.5, with a single maximum at 5.9. The activity is rapidly inactivated below pH 5.0. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles . |
| Legal Information: | A product of Novozyme Corp. |
| Legal Information: | BAN is a trademark of Novozymes Corp. |
| Unit Definition: | One unit is the amount of enzyme which dextrinizes 5.26 g of dry starch per hour under standard conditions. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P304 + P340 + P312 - P501 |
| Hazard Codes | Xn |
| Risk Statements | 42 |
| Safety Statements | 36/37/39 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| activity | specific activity: ≥250 units/g |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.1. |
| UNSPSC | 12352204 |