Monoclonal Anti-Actin, Smooth Muscle antibody produced in mouse
SIGMA/A7607 - ~1.0 mg/mL, clone CGA7, purified immunoglobulin, buffered aqueous solution
MDL Number: MFCD00162107
Product Type: Chemical
Immunofluorescence Mouse colon sections, paraffin embedded-ethanol fixed were stained with 10 μg/mL of Monoclonal Anti-Actin, smooth Muscle Specific (Cat. No. A7607). The antibody was developed with Goat Anti-Mouse IgG, FITC conjugate (Cat. No. F8771).
Western Blotting Whole extract of Chicken gizzard were separated on SDS-PAGE and probed with Monoclonal Anti-Actin, Smooth Muscle antibody produced in Mouse, Clone: CGA7 (Cat. No. A7607). The antibody was developed using 1:5,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A2304). Lanes 1. 10 μg/mL antibody 2. 5 μg/mL antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CGA7, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | antigen ~42 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, chicken, mouse, human, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| western blot: 5-10 μg/mL using chicken gizzard total extract | |
| UniProt accession no. | P62736  |
| Application: | Monoclonal Anti-Actin, Smooth Muscle antibody produced in mouse has been used in: • enzyme-linked immunosorbent assay (ELISA) • immunoblotting • immunocytochemistry • immunohistochemistry |
| Biochem/physiol Actions: | Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Mutations in alpha-actin 2 (ACTA2) results in thoracic aortic aneurysm, Moyamoya disease, coronary artery disease, stroke and multisystemic smooth muscle dysfunction syndrome. It plays a functional role in the smooth muscle of non-vascular tissues. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. Its expression is related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha actins (skeletal, cardiac, smooth), one beta actin (beta-non-muscle) and two gamma actins (gamma smooth muscle and gamma non-muscle). Monoclonal Anti-Actin, Smooth Muscle, reacts with human, monkey, chicken, rat, and mouse smooth muscle actin. |
| General description: | Monoclonal Anti-Actin, Smooth Muscle (mouse IgG2b isotype) is derived from the hybridoma CGA7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with partially purified chicken gizzard actin. α-actin 2 (ACTA2) codes for a smooth muscle specific α-actin. |
| General description: | The antibody specifically recognizes α and γ smooth muscle actin. |
| Immunogen: | partially purified chicken gizzard actin. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |