Apyrase from potatoes
SIGMA/A7646 - ATPase ≥60 units/mg protein, lyophilized powder (partially purified)
Synonym: Adenosine 5′-diphosphatase; Adenosine 5′-triphosphatase
CAS Number: 9000-95-7
EC Number: 232-569-8
MDL Number: MFCD00130542
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A7646-2KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A7646-5KU
| application(s) | diagnostic assay manufacturing |
| ATPase activity | ≥60 units/mg protein |
| biological source | potato |
| composition | Protein, 30-60% |
| foreign activity | Acid Phosphatase ≤5% of base activity |
| form | lyophilized powder (partially purified) |
| mol wt | ~49 kDa by gel filtration |
| Quality Level | 200  |
| solubility | H2O: soluble 10 mg/mL, slightly hazy |
| storage temp. | −20°C |
| type | Grade III |
| Application: | Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1 Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation. The enzyme has been used to assess the possibility of ATP involvement in intestinal lamina propria Th17 cell differentiation. |
| Application: | At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1). Reaction: ATP → ADP+Pi → AMP+2Pi. |
| Biochem/physiol Actions: | Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM. The molecular weight of the protein is found to be approximately 45 kDa. |
| Other Notes: | Mixture of high and low ratio isoenzymes. |
| Packaging: | 100, 200 units in glass bottle |
| Packaging: | 500, 1000, 2000, 5000 units in poly bottle |
| Packaging: | Sold on the basis of ATPase units |
| Quality: | Contains ≤ 5% acid phosphatase. |
| Unit Definition: | One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | ATPase activity: ≥60 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.6.1.5 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |