Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit
SIGMA/A7650 - affinity isolated antibody, buffered aqueous solution
Synonym: Rabbit Anti-Goat IgG (whole molecule)–AP
MDL Number: MFCD00162335
Product Type: Chemical
ELISA Anti-Goat IgG (whole molecule)-Alkaline Phosphatase antibody produced in Rabbit (Cat. No. A7650) was serially diluted to detect Goat IgG by direct ELISA.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:7,000 |
| Application: | Anti-Goat IgG (whole molecule)-Alkaline Phosphatase antibody produced in rabbit has been used in immunoblotting and enzyme-linked immunosorbent assay (ELISA). |
| Application: | Anti-Goat IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit has been used in western blotting. |
| Application: | Immunocytochemistry was performed on cells fixed in 4% paraformaldhyde for 15 minutes using alkaline phosphatase-conjugated rabbit anti-goat IgG diluted 1:1000 in TBS as the secondary antibody. |
| Biochem/physiol Actions: | IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to the fetus through the placenta that is vital for immune defense of the neonate against infections. |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) mainly helps in immune defense.(5) IgG participates in hypersensitivity type II and type III. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | An immunoglobulin has two heavy chains and two light chains connected by disulfide bond.(5) It is a glycoprotein.IgG is a major class of immunoglobulin.(6) Goat IgG has two subclasses- IgG1 and IgG2.(7) |
| General description: | Second antibodies (secondary antibodies) are antibodies raised against other antibodies, typically from another species, called primary antibodies. Primary antibodies are generated with and bind to specific antigens to create antigen antibody complexes which may be detected in a variety of immunochemical and immunohistochemical configurations involving soluble or insoluble signaling molecules. The immunodetection system depends upon the binding of a signal competent secondary antibody to a primary antibody:antigen complex. The suitability of any secondary antibody to a specific immunodetection application is dependent upon its signal development system configuration. Signal development systems are based upon the chemical conjugation of a signal mediator to the secondary antibody. Major secondary antibody signal systems are color (chromogenic), chemiluminescence or fluorescence based and involve the use of enzymes or high affinity binary systems such as biotin:avidin. |
| Immunogen: | Antiserum is produced in rabbit using purified goat IgG as the immunogen |
| Physical form: | Solution in 0.05 M Tris, pH 8.0, containing 10 mM glycine, 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide. |
| Specificity: | Antiserum is determined to be immunospecific for goat IgG by immunoelectrophoresis (IEP), prior to conjugation, versus goat IgG and normal goat serum. |
| Storage and Stability: | Store at 2-8°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |