L-Alanine Dehydrogenase from Bacillus subtilis
SIGMA/A7653 - buffered aqueous glycerol solution, ~30 units/mg protein (Lowry)
Synonym: L-Alanine: NAD+ oxidoreductase (deaminating)
CAS Number: 9029-06-5
EC Number: 232-847-9
MDL Number: MFCD00131441
Product Type: Chemical
Unit Definition One unit will convert 1.0 μmole of L-alanine to pyruvate and NH3 per min at pH 10.0 at 25 °C. β-NAD = β-Nicotinamide Adenine Dinucleotide, Oxidized Form. β-NADH = β-Nicotinamide Adenine Dinucleotide, Reduced Form.
| biological source | Bacillus subtilis |
| foreign activity | LDH ~1% (using pyruvate as substrate) |
| form | buffered aqueous glycerol solution |
| Quality Level | 200  |
| specific activity | ~30 units/mg protein (Lowry) |
| storage temp. | −20°C |
| Application: | L-Alanine dehydrogenase converts L-alanine to pyruvate and ammonium. L-Alanine dehydrogenase from Bacillus subtilis may be used to study enzyme inactivation and protection . |
| Biochem/physiol Actions: | L-Alanine dehydrogenase is an A-stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the generation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate, and NADH are released in that specific order. Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent metal ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L- or D-cysteine. |
| Packaging: | 100 units in glass bottle |
| Physical form: | Solution in 50% glycerol containing 10 mM potassium phosphate buffer, pH 7.7 |
| Unit Definition: | One unit will convert 1.0 μmole of |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P304 + P340 + P312 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| activity | specific activity: ~30 units/mg protein (Lowry) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.4.1.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |