Monoclonal Anti-α-Actinin (Sarcomeric) antibody produced in mouse
SIGMA/A7811 - clone EA-53, ascites fluid
Synonym: Actinin Antibody; Sarcomeric Alpha Actinin Antibody; Sarcomeric Alpha Actinin Antibody - Monoclonal Anti-α-Actinin (Sarcomeric) antibody produced in mouse; Anti-1110008F24Rik; Anti-ACTN2; Anti-Actinin α2; Anti-CMD1AA; Anti-MGC107582
MDL Number: MFCD00164521
Product Type: Chemical
Immunohistochemistry JOURNAL CITATION: Knockdown and overexpression of Unc-45b result in defective myofibril organization in skeletal muscles of zebrafish embryos. By: Bernick, E. P., Zhang, P. J., et al. in BMC Cell Biol, 2010. PubMed ID: 20849610 Image collected and cropped by CiteAb from the following publication, (http://bmccellbiol.biomedcentral.com/articles/10.1186/1471-2121-11-70), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry JOURNAL CITATION: Knockdown and overexpression of Unc-45b result in defective myofibril organization in skeletal muscles of zebrafish embryos. By: Bernick, E. P., Zhang, P. J., et al. in BMC Cell Biol, 2010. PubMed ID: 20849610 Image collected and cropped by CiteAb from the following publication, (http://bmccellbiol.biomedcentral.com/articles/10.1186/1471-2121-11-70), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry JOURNAL CITATION: Knockdown and overexpression of Unc-45b result in defective myofibril organization in skeletal muscles of zebrafish embryos. By: Bernick, E. P., Zhang, P. J., et al. in BMC Cell Biol, 2010. PubMed ID: 20849610 Image collected and cropped by CiteAb from the following publication, (http://bmccellbiol.biomedcentral.com/articles/10.1186/1471-2121-11-70), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry
Staining of formalin-fixed, paraffin-embedded section of human tongue using Monoclonal Anti-α-Actininn (Cat. No. A7811) showing specific staining of striated muscle. Antibody dilution 1:800.
Staining of formalin-fixed, paraffin-embedded section of human tongue using Monoclonal Anti-α-Actininn (Cat. No. A7811) showing specific staining of striated muscle. Antibody dilution 1:800.
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1:500 Monoclonal Anti-α-Actinin (Sarcomeric) Clone: EA-53 (Cat. No. A7811). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Double immunofluorescent labeling of chicken heart cells with Monoclonal Anti-α-Actinin (Sarcomeric), Clone No. EA-53 (Cat. No. A7811) (red) and FITC-phalloidin (green). Notice the sarcomeric structure in the muscle cell at the center of the micrograph and the stress fibers in the adjacent fibroblasts. Superimposed images are shown on the right.
From Prof. B Geiger and T. Volberg, Weizmann Institute of Science, Rehovot, Israel.
From Prof. B Geiger and T. Volberg, Weizmann Institute of Science, Rehovot, Israel.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:1,000 Monoclonal Anti-α-Actinin (Sarcomeric) Clone: EA-53 (Cat. No. A7811). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Rat skeletal muscle 2. Rat heart 3. C2C12 4. Differentiated C2C12
Immunoblotting Whole extract of rat leg muscle was separated on SDS-PAGE and probed with Monoclonal Anti-α-Actinin (Sarcomeric) Clone: EA-53 (Cat. No. A7811). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:2,500 2. Antibody dilution 1:5,000
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | EA-53, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 100 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, frog, pig, feline, chicken, hamster, canine, bovine, fish, snake, rabbit, sheep, goat, rat, lizard |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800 using human skeletal and cardiac muscle |
| indirect ELISA: suitable using human and animal muscle tissue | |
| indirect immunofluorescence: suitable using human and animal cultured muscle cells | |
| western blot: 1:2,500 using rat leg muscle | |
| UniProt accession no. | P35609  |
| Application: | Cardiac myocytes were stained with monoclonal mouse anti-actinin to visualize the Z-lines after fixation in pre-cooled methanol/acetone mix (1:1) for 10 minutes at 4 °C. |
| Application: | Mouse monoclonal anti-α-actinin (sarcomeric) antibody is suitable for immunohistochemistry (1:800), ELISA, immunofluorescence and western blot (1:2,500) applications. |
| Biochem/physiol Actions: | α-actinin functions as an actin crosslinker and promotes cell migration. Mutations in α-actinin gene locus is observed in patients with heterogeneous hypertrophic cardiomyopathy and in juvenile onset atrial fibrillation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | α-Actinin (ACTN2) is a 100kDa actin-binding protein that is found in muscle as well as non-muscle cells. In smooth muscles, α-actinin is present in dense bodies and plaques whereas in normal skeletal muscles, it is associated with z-discs that define muscle sarcomeres. ACTN2 is located on human chromosome lq42-q43. α-actinin has rod shaped N-terminal domain. Mouse monoclonal anti-α-actinin (sarcomeric) antibody stains thymic myoid cells. The antibody exhibits wide reactivity with human and animal muscle tissue. The antibody is specific for a-skeletal and a-cardiac muscle actinins. The antibody labels Z lines and dots in stress fibers of skeletal muscle in myotubes but does not react with non-skeletal muscle elements (e.g., connective tissue, epithelium, nerves, smooth muscle). Mouse monoclonal anti-α-actinin (sarcomeric) antibody stains thymic myoid cells. The antibody exhibits wide reactivity with human and animal muscle tissue. α-actinin is detected predominantly in dense bodies and plaques which are characteristic of that tissue. Immunofluorescent labeling of a large variety of cells with anti a -actinin reveals an extensive association of the proteins with the actin containing stress fibers and, in particular, with their membrane-bound termini. Monoclonal Anti-α-Actinin (Sarcomeric) (mouse IgG1 isotype) is derived from the EA-53 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rabbit skeletal a-actinin.1 The isotype is determined using Mouse Monoclonal Antibody Isotyping Reagents, Catalog Number ISO2. Monoclonal Anti-α-Actinin (Sarcomeric) may be used for the localization of sarcomeric α-actinin using various immunochemical assays such as ELISA, dot blot, immunoblot, immunohistochemistry, and immunocytochemistry. The antibody is useful in the immunolocalization of α-actinin in normal and neoplastic cultured cells and tissues, and for studies on the state of sarcomeric muscle organization, in normal and pathological situations. |
| Immunogen: | Rabbit skeletal α-actinin |
| Physical form: | Supplied as ascites fluid with 15 mM sodium azide as a preservative |
| Specificity: | The antibody is specific for a-skeletal and α-cardiac muscle actinins. The antibody labels Z lines and dots in stress fibers of skeletal muscle in myotubes but does not react with non-skeletal muscle elements (e.g., connective tissue, epithelium, nerves, smooth muscle). |
| Storage and Stability: | Store at -20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots at -20 °C. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |