Anti-Goat/Sheep IgG−Alkaline Phosphatase antibody, Mouse monoclonal
SIGMA/A8062 - clone GT-34, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Goat/Sheep IgG; Monoclonal Anti-Goat/Sheep IgG–Alkaline Phosphatase antibody produced in mouse
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human tonsil tissue sections were stained with 1:100 Anti-Human IgG (whole molecule) antibody produced in Goat (Cat. No. I1011) followed by 1:80 Monoclonal Anti-Goat/Sheep IgG-Alkaline Phosphatase antibody produced in Mouse, Clone: GT-34 (Cat. No. A8062).
Western Blotting Whole extract of Chicken fibroblasts cells were separated on SDS-PAGE and probed with 1:1,000 Anti-Filamin antibody produced in Goat. The antibody was developed using Monoclonal Anti-Goat/Sheep IgG-Alkaline Phosphatase antibody produced in Mouse, Clone: GT-34 (Cat. No. A8062). Lanes 1. 150,000 antibody 2. 300,000 antibody 3. Negative Control
ELISA Monoclonal Anti-Goat/Sheep IgG-Alkaline Phosphatase antibody produced in Mouse, Clone: GT-34 (Cat. No. A8062), was serially diluted to detect Goat/Sheep IgG by direct ELISA. Reactivity is observed with Goat IgG, Sheep IgG and Bovine IgG; but not with Human IgG.
| antibody form | purified from hybridoma cell culture |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | GT-34, monoclonal |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous glycerol solution |
| isotype | IgG1 |
| shipped in | wet ice |
| should not react with | rat, canine, mouse, rabbit, guinea pig, chicken, horse, pig, feline, human |
| species reactivity | sheep, goat, bovine |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:50,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 | |
| western blot (chemiluminescent): 1:150,000 using total cell extract of chicken fibroblasts (10-20 μg per well) |
| Application: | Mouse monoclonal anti-goat/sheep IgG-alkaline phosphatase antibody has been used as a secondary antibody for ELISA assays at 1:4,000 and 1:15,210 dilutions. The product can also be used for IHC (1:40) and western blot applications (1:150,000). |
| Application: | The level of IL-5 in HPAEC conditioned medium was determined by ELISA using alkaline phosphatase-conjugated monoclonal mouse anti-goat/sheep IgG (GT-34) as the secondary antibody. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgGs are glycoprotein antibodies that modulate several immune responses. Primary goat against target proteins are often used in various research applications. Thus, secondary anti-goat IgGs can be useful tools for the analysis of target proteins. However, secondary antibodies may lack species specificity as they may bind to non-related Igs in the test preparation. This may lead to increased levels of background staining. Hence, use of monoclonals can effectively reduce background staining and increase the specificity of immunoassays. Monoclonal Anti-Goat/Sheep IgG-Alkaline Phosphatase recognizes goat and sheep IgGs. No cross-reactivity is observed with human IgGs or IgGs derived from the following species: guinea pig, rat, horse, dog, chicken, pig, mouse, rabbit, and cat. |
| General description: | The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2. |
| Immunogen: | Purified goat IgG |
| Physical form: | Solution in 0.05 M Tris buffer pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |