Anti-hABH1 antibody, Mouse monoclonal
SIGMA/A8103 - clone hABH1-151, purified from hybidoma cell culture
Synonym: Anti-Human AlkB Homologue 1; Monoclonal Anti-hABH1 antibody produced in mouse
MDL Number: MFCD06412250
Product Type: Chemical
Immunoblotting 293T cell extract was separated on SDS-PAGE and probed with Monoclonal PA to HABH1 (MO) CL: HABH1-151 (Cat. No. A8103). The antibody was developed with Biotin APA M IgG (Fab+Fc) kit comp. (Cat. No. B6398), Alk Phos ExtrAvidin (Cat. No. E2636) and NBT/BCIP substrate. Antibody concentration 2 μg/mL.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | hABH1-151, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~45 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1-2 μg/mL using total cell extract of 293T cells | |
| UniProt accession no. | Q13686  |
| Application: | Anti-hABH1 antibody, Mouse monoclonal has been used in • chromatin immunoprecipitation(ChIP) • immunohistochemistry • enzyme-linked immunosorbent assay (ELISA) • immunoblotting • immunoprecipitation |
| Biochem/physiol Actions: | The human homolog, AlkB homolog 1, histone H2A dioxygenase (Alkbh1) is linked with several enzymatic actions, such as, DNA, mRNA or tRNA hydroxylation, cleavage at abasic (AP) site in DNA and demethylation of histones. It plays a key role in epigenetic gene silencing, as it is known to exhibit m6A demethylation activity . ALKBH1 participates in normal cell proliferation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-hABH1 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hABH1-151 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human hABH1, conjugated to keyhole limpet hemocyanin (KLH). |
| General description: | Human AlkB homologue 1 (hABH1) is one of the eight homologs of E. coli AlkB. Homology modeling studies have analyzed the role of hABH1 gene in DNA repair after alkaylation-induced damage. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Monoclonal Anti-hABH1 (human AlkB homologue) recognizes human and monkey ABH1. It does not recognize hABH2 or hABH3. The antibody epitope resides within amino acids 19-34 of human hABH1. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |