Amido Black Staining Solution 2X
SIGMA/A8181 - electrophoresis reagent
Synonym: Naphthol Blue Black solution; Amido Black 10B
CAS Number: 1064-48-8
Empirical Formula (Hill Notation): C22H14N6Na2O9S2
Molecular Weight: 616.49
MDL Number: MFCD00004017
Linear Formula: C22H14N6Na2O9S2
Product Type: Chemical
| form | liquid |
| InChI | 1S/C22H16N6O9S2.2Na/c23-1 |
| InChI key | AOMZHDJXSYHPKS-DROYEMJCSA |
| Quality Level | 200 ![]() |
| SMILES string | [Na+].[Na+].Nc1c(N=N/c2c |
| Application: | Amido Black Staining Solution 2X has been used in the staining: • serum proteins present in the culture supernatant • cysteine sulfinic acid decarboxylase protein in mouse liver homogenate • histones from chromatin samples |
| General description: | Amido black (AB) is an acidic dye used for staining proteins. It can detect proteins levels of 50ng in both polyvinylidene difluoride (PVDF) and nitrocellulose membrane. AB aids protein visualization even at low concentrations. It has applications in forensics due to its ability to stain blood proteins in fingerprints. |
| Preparation Note: | After dilution with deionized water to a final volume of 500 ml, solution will contain 0.1% (w/v) amido black, 25% (v/v) isopropanol and 10% (v/v) acetic acid. |
| Symbol | ![]() GHS02,GHS07 |
| Signal word | Danger |
| Hazard statements | H225 - H315 - H319 - H336 |
| Precautionary statements | P210 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 10-36/38-67 |
| Safety Statements | 26 |
| RIDADR | UN 2924 8(3) / PGIII |
| WGK Germany | WGK 2 |
| UNSPSC | 12171500 |



