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Amido Black Staining Solution 2X

SIGMA/A8181 - electrophoresis reagent

Synonym: Naphthol Blue Black solution; Amido Black 10B

CAS Number: 1064-48-8
Empirical Formula (Hill Notation): C22H14N6Na2O9S2
Molecular Weight: 616.49
MDL Number: MFCD00004017
Linear Formula: C22H14N6Na2O9S2
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-A8181-1EA 1 ea
$141.00
1/EA
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form liquid
InChI 1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;
InChI key AOMZHDJXSYHPKS-DROYEMJCSA-L
Quality Level 200 
SMILES string [Na+].[Na+].Nc1c(N=N/c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(C=C/c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O
Application: Amido Black Staining Solution 2X has been used in the staining:
• serum proteins present in the culture supernatant
• cysteine sulfinic acid decarboxylase protein in mouse liver homogenate
• histones from chromatin samples
General description: Amido black (AB) is an acidic dye used for staining proteins. It can detect proteins levels of 50ng in both polyvinylidene difluoride (PVDF) and nitrocellulose membrane. AB aids protein visualization even at low concentrations. It has applications in forensics due to its ability to stain blood proteins in fingerprints.
Preparation Note: After dilution with deionized water to a final volume of 500 ml, solution will contain 0.1% (w/v) amido black, 25% (v/v) isopropanol and 10% (v/v) acetic acid.
UNSPSC 12171500

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