Aminopeptidase from Aeromonas proteolytica
SIGMA/A8200 - lyophilized powder, 50-150 units/mg protein
Synonym: AAP; Aminopeptidase from Vibrio proteolyticus; bacterial leucyl aminopeptidase
CAS Number: 37288-67-8
EC Number: 232-874-6
MDL Number: MFCD00166325
Product Type: Chemical
Unit Definition One unit will hydrolyze 1.0 μmole of L-leucine p-nitroanilide to L-leucine and p-nitroaniline per min at pH 8.0 at 25 °C.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A8200-100UN
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A8200-250UN
| composition | Protein, ~40% biuret |
| foreign activity | endopeptidase, essentially free |
| form | lyophilized powder |
| grade | Proteomics Grade |
| mol wt | 29.5 kDa |
| Quality Level | 300  |
| solubility | H2O: soluble 0.9-1.1 mg/mL, clear, colorless |
| specific activity | 50-150 units/mg protein |
| storage temp. | −20°C |
| Application: | Aminopeptidases are a family of widely distributed proteases, which may be used to study many significant biological processes such as protein maturation, hormone production, and peptide digestion. The enzyme has been used to measure the kinetic rate constant for the binding of bestatin, a general protease inhibitor, to aminopeptidase. |
| Biochem/physiol Actions: | Aminopeptidase from Aeromonas proteolytica is a metalloenzyme, which contains 2 atoms of Zn2+ in a single polypeptide with an approximate molecular weight of 29.5 kDa as determined by sedimentation. This enzyme has a high degree of stability, being stable even at temperatures of 70 °C for several hours. Partial inactivation occurs in 8 M urea. Maximum stability and activity are between pH 8.0-8.5. Aminopeptidase from Aeromonas proteolytica can function as an esterase. |
| Biochem/physiol Actions: | Aminopeptidase from Aeromonas proteolytica is involved in protein maturation, hormone production and peptide digestion. |
| Biochem/physiol Actions: | Catalyzes the release of an |
| General description: | A zinc-containing enzyme. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of |
| Packaging: | 100, 250 units in glass bottle |
| Physical form: | Lyophilized powder containing tricine buffer, pH 8.0, zinc chloride and stabilizer. |
| Preparation Note: | Dissolves in water at 0.9-1.1 mg/mL concentration to form a clear, colorless solution. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 50-150 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.11.10 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |