Anti-hABH2 antibody, Mouse monoclonal
SIGMA/A8228 - clone hABH2-7, purified from hybridoma cell culture
Synonym: Anti-Human AlkB Homologue 2; Monoclonal Anti-hABH2 antibody produced in mouse
MDL Number: MFCD06412251
Product Type: Chemical
Immunoblotting 293T cell extract was separated on SDS-PAGE and probed with Monoclonal PA to HABH2 (MO) CL: HABH2-7 (Cat. No. A8228). The antibody was developed with Biotin APA M IgG (Fab+Fc) kit comp. (Cat. No. B6398), Alk Phos ExtrAvidim (Cat. No. E2636) and NBT/BCIP substrate. Antibody dilution 2 μg/mL.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | hABH2-7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~30 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1-2 μg/mL using total cell extract of 293T cells | |
| UniProt accession no. | Q6NS38  |
| Application: | Mouse Monoclonal Anti-hABH2 antibody has been used for western blot assays. The antibody can also be used for IHC, ELISA, microarray, and immunoprecipitation studies. |
| Biochem/physiol Actions: | hABH2 is a Fe (II) dependent dioxygenase involved in the repair of alkylation-induced damage in double stranded DNA. Alterations in hABH2 have been linked to pediatric brain tumor and gastric cancer. |
| Biochem/physiol Actions: | Removal of the methyl groups of 1-methyladenine (1-meA) and 3-methylcystosine (3-meC) to their unmodified forms is done by human alkB homolog 1, histone H2A dioxygenase (hABH2) and hABH3. hABH2 acts on double stranded DNA. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-hABH2 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hABH2-7 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide of human hABH2, conjugated to KLH. Human alkB homolog 1, histone H2A dioxygenase (hABH2) shares homology with the AlkB protein in bacteria. hABH2 localizes to the nucleoplasm and occasionally to the nucleoli. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |