Anti-α-Amylase antibody produced in rabbit
SIGMA/A8273 - fractionated antiserum, lyophilized powder
Synonym: Alpha Amylase Antibody; Alpha Amylase Antibody - Anti-α-Amylase antibody produced in rabbit
MDL Number: MFCD00162394
Product Type: Chemical
Immunohistochemistry JOURNAL CITATION: Dicer is required for maintenance of adult pancreatic acinar cell identity and plays a role in Kras-driven pancreatic neoplasia. By: Wang, Y. J., McAllister, F., et al. in PLoS One, 2014. PubMed ID: 25405615 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0113127), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
ELISA Anti-α-Amylase antibody produced in Rabbit (Cat. No. A8273), followed by 1:1,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545), was serially diluted to detect ALPHA-Amylase by indirect ELISA.
| antibody form | fractionated antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| packaging | vial of 2 mL lyophilized antiserum |
| Quality Level | 200  |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:4,000-1:6,000 |
| Ouchterlony double diffusion: suitable |
| Application: | Anti-α-Amylase antibody produced in rabbit has also been used in immunocytochemistry analysis. |
| Application: | Anti-a-Amylase antibody produced in rabbit has been used in immunofluorescence and immunohistochemical analysis. |
| Application: | Mouse pancreatic sections were fixed in 4% paraformaldehyde and used for immunohistochemistry using rabbit anti-amylase antibody at a dilution of 1:100. |
| Biochem/physiol Actions: | α-Amylase is essential for catalyzing the primary step in starch digestion, a main source of carbohydrate in the human diet. α-amylase hydrolysis the polysaccharide with the ultimate production of maltose, maltotriose and limit dextrins as the main products. |
| Biochem/physiol Actions: | The antiserum is specific for human a-amylase found in human saliva and human pancreatic extract. No reaction with other human saliva proteins or pancreatic extract proteins is observed. |
| General description: | α-amylase is primarily produced in abundance in the salivary glands and pancreas. The expression is also seen in jejunum and mammary glands. The genes encoding α-amylase are AMY1 and AMY2 located in the short arm of chromosome 1. AMY1 is responsible for producing the enzyme in saliva and mammary gland and AMY2 produces the enzyme synthesized in the pancreas. The expression of AMY1 occurs also in certain tumour tissues. Mammalian amylases are composed of three structural domains (A, B and C). Domain A contains the active site with two aspartate and one glutamate residue. Domain B borders the active site region and is essential for maintaining the protein conformation. Domain C is involved in catalytic mechanism. |
| Immunogen: | human salivary α-amylase. |
| Physical form: | Lyophilized from 0.01 M phosphate buffered saline, pH 7.2 |
| Preparation Note: | Reconstitute with 2 mL deionized water. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |