Antifoam 204
SIGMA/A8311 - aqueous emulsion for bacterial and mammalian systems
Synonym: organic antifoam
MDL Number: MFCD00130523
Product Type: Chemical
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| application(s) | microbiology |
| concentration | 100% (organic) |
| density | 1.01 g/mL at 25 °C |
| form | emulsion (aqueous) |
| Quality Level | 200  |
| storage temp. | room temp |
| suitability | (Mammalian (suspension)) |
| bacteria (fermentation) |
| Application: | Suitable for preventing or minimizing foaming in microbial fermentation systems and mammalian suspension cultures. Antifoam 204 has been used: • in the expression and purification of PUL (PLAP, Ufd3p, and Lub1p) domain • in fed-batch fermentation • to prevent bubble formation due in aeration in Luria Bertani (LB) culture medium for bacterial growth • in the batch fermentation with mineral medium |
| General description: | Antifoam 204 is an organic antifoam used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production. |
| Other Notes: | Antifoam 204 is a 100% active components and a mixture of non-silicone organic defoamers in a polyol dispersion. |
| Other Notes: | Contains 100% active components and is a mixture of non-silicone organic defoamers in a polyol dispersion. Can be sterilized repeatedly. |
| Preparation Note: | For use in microbiological media Sigma recommends a starting concentration of between 0.005% and 0.01%. The optimal amount of antifoam required for various applications will need to be determined. Antifoam 204 is soluble in methanol, ethanol, toluene, xylene, perchloroethylene, and cold water at temperatures below 15 °C. It is insoluble in warm water and ethylene glycol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Density | 1.01 g/mL at 25 °C |
| Storage Temp. | room temp |
| UNSPSC | 12161901 |