m-Aminophenylboronic acid–Agarose
SIGMA/A8312 - aqueous suspension
Synonym: (3-
MDL Number: MFCD00801303
Product Type: Chemical
| capacity | ≥8 mg/mL binding capacity (peroxidase Type VI) |
| extent of labeling | 40-80 μmol per mL |
| form | aqueous suspension |
| matrix | 6% beaded agarose |
| matrix activation | epichlorohydrin |
| matrix attachment | through amino to carboxyls of EDTA |
| matrix spacer | 9 atoms |
| Quality Level | 300  |
| SMILES string | [X]Nc1cc(ccc1)B(O)O |
| storage temp. | 2-8°C |
| Application: | m-Aminophenylboronic acid-Agarose has been used: • as a component of the column for the purification of Escherichia coli TOP10F cells • with cell lysates of various cells for detection of PARylated (Poly(ADP-ribos)ylation) proteins. • in the affinity purification of horseradish peroxidase (HRPO) • in the glycated human serum albumin (gHSA) purification |
| General description: | m-Aminophenylboronic acid (m-APBA) immobilized on an agarose gel is useful in immunoglobulins capture, but also leads to non-specific interactions. m-APBA is a boronate affinity matrix that is effectively used for affinity purification of monoclonal antibodies. |
| Physical form: | Suspension in water containing 0.002% chlorhexidine diacetate. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12161501 |