Anti-β-Amyloid Protein (1-40) antibody produced in rabbit
SIGMA/A8326 - whole antiserum
Synonym: Anti-AAA; Anti-ABETA; Anti-ABPP; Anti-AD1; Anti-APPI; Anti-CTFgamma; Anti-CVAP; Anti-PN-II; Anti-PN2; Anti-alpha-sAPP; Anti-preA4
MDL Number: MFCD00212892
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Formalin fixed, paraffin-embedded, methacarn-treated sections of Human Alzheimer’s disease (AD) brain were stained with 1:100 Anti-β-Amyloid Protein (1-40) antibody produced in Rabbit (Cat. No. A8326) (A) and developed using 15 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886), and 2.5 μg/mL Anti-β-Amyloid antibody, Mouse monoclonal, Clone: BAM-10 (Cat. No. A3981) (B). The antibody was developed using using 15 μg/mL Anti-Mouse IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B7151) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886). Results Two Anti-β-Amyloid antibodies,A8326 (A) and A3981 (B) target different regions of β-Amyloid show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Formalin fixed, paraffin-embedded, methacarn-treated sections of Human Alzheimer’s disease (AD) brain were stained with 1:100 Anti-β-Amyloid Protein (1-40) antibody produced in Rabbit (Cat. No. A8326), followed by 15 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunoblotting Beta-Amyloid (Sig. A5813) was separated on SDS-PAGE and probed with AB to Beta-Amyloid (1-40) (RB) (Prepack) (Cat. No. A8326). The antibody was developed with Alk Phos APA Rabbit Igc (Cat. No. A9919) and NBT/BCIP substrate. Antibody dilution 1/1,000.
| antibody form | whole antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | liquid |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human Alzheimer’s disease (AD) brain tissue |
| indirect ELISA: 1:4000-1:8000 | |
| UniProt accession no. | P05067  |
| Application: | Anti-β-Amyloid Protein (1-40) antibody produced in rabbit has been used in: • immunocytochemical localization of Aβ peptides • immunocytochemistry • immunoprecipitation • focused ultrasound-microbubble enhanced antibody delivery (FUS-MB) |
| Biochem/physiol Actions: | The β-amyloid precursor protein (APP) is cleaved sequentially by the proteolytic enzymes β-secretase (BACE1) and γ-secretase to produce β-amyloid (Aβ) peptides with the Aβ1-42 and the Aβ1-40 forms being the most prevalent. Secreted Aβ peptides are degraded either via a re-uptake mechanism followed by endosomal degradation, or by an extracellular insulin degrading enzyme. Extracellular accumulation of Aβ leads to the formation of aggregates, fibrils and eventually amyloid deposits called neuritic plaques, which is the hallmark of Alzheimer′s disease (AD). Rabbit Anti-β-Amyloid Protein (1-40) antibody does not stain control sections of normal brain tissues. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Amyloid precursor proteins (APPs) are members of a large family of 70 kDa transmembrane glycoproteins that are found in a wide range of tissues. APP is expressed in the brain. It is located on human chromosome 21. APPs have three main isoforms, namely, APP695, APP751 and APP770, that are derived from alternative splicing events in cells. |
| Immunogen: | synthetic β-amyloid (1-40) conjugated to BSA. |
| Physical form: | Rabbit Anti-β-Amyloid (1-40) is supplied as a liquid containing 0.1% sodium azide as preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |