Anti-hABH3 antibody, Mouse monoclonal
SIGMA/A8353 - clone hABH3-99, purified from hybridoma cell culture
Synonym: Anti-Human AlkB Homologue 3; Monoclonal Anti-hABH3 antibody produced in mouse
MDL Number: MFCD06412252
Product Type: Chemical
Immunoblotting 293T cell extract was separated on SDS-PAGE and probed with Monoclonal PA to HABH3 (MO) CL: HABH3-99 (Cat. No. A8353). The antibody was developed with Biotin APA M IgG (Fab+Fc) kit comp. (Cat. No. B6398), Alk Phos ExtrAvidim (Cat. No. E2636) and NBT/BCIP substrate. Antibody dilution 2 μg/mL.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | hABH3-99, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~33 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, hamster, monkey, bovine, canine, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1-2 μg/mL using total cell extract of 293T cells | |
| UniProt accession no. | Q96Q83  |
| Application: | • Mouse Monoclonal Anti-hABH3 has been used in: • western blot • immunohistochemistry • immunoprecipitation • indirect enzyme linked immunosorbent assay (ELISA) |
| Application: | Mouse Monoclonal Anti-hABH3 can be used for western blot (1-2μg/mL), immunohistochemistry, immunoprecipitation, indirect ELISA and microarray studies. |
| Biochem/physiol Actions: | hABH3 is a Fe(II)-dependent dioxygenase involved in the repair of alkylation-induced damage in DNA. hABH3 mutations have been analysed in pediatric brain tumors. Mouse Monoclonal Anti-hABH3 binds to human, monkey, bovine, dog, rat, hamster, and mouse hABH3. It does not recognize hABH1 or hABH2. The antibody epitope resides within amino acids 2-16 of human hABH3. |
| Biochem/physiol Actions: | Human alkB homolog 3, α-ketoglutarate dependent dioxygenase (hABH3) is involved in the repair of alkylated RNA. This antibody serves as an important tool for studying the regulation of DNA/RNA repair enzymes. |
| Biochem/physiol Actions: | Mouse Monoclonal Anti-hABH3 binds to human, monkey, bovine, dog, rat, hamster, and mouse hABH3. It does not recognize hABH1 or hABH2. The antibody epitope resides within amino acids 2-16 of human hABH3. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Human alkB homolog 3, alpha-ketoglutarate dependent dioxygenase (hABH3) is mainly found in the nucleoplasm and cytoplasm. Anti-hABH3 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hABH3-99 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with hABH3, conjugated to keyhole limpet hemocyanin (KLH). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| WGK Germany | - |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |