Monoclonal Anti-α-Fetoprotein (AFP) antibody produced in mouse
SIGMA/A8452 - ascites fluid, clone C3
Synonym: Afp Antibody; Afp Antibody - Monoclonal Anti-α-Fetoprotein (AFP) antibody produced in mouse; Alpha Fetoprotein Antibody
MDL Number: MFCD00162111
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Cardiogenic programming of human pluripotent stem cells by dose-controlled activation of EOMES. By: Pfeiffer, M. J., Quaranta, R., et al. in Nat Commun, 2018. PubMed ID: 29382828 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/s41467-017-02812-6), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Formalin-fixed, paraffin-embedded section of human fetal liver stained with 1:500 Monoclonal Anti-α-Fetoprotein (AFP) antibody produced in Mouse clone C3 (Cat. No. A8452) followed by Anti-Mouse IgG (Fab specific)-Biotin antibody produced in Goat (Cat. No. B0529) and by ExtrAvidin®-Peroxidase (Cat. No. E2886).
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | C3, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2a |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | mouse, cat, rat, bovine |
| species reactivity | pig, canine, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human fetal liver | |
| indirect ELISA: suitable | |
| UniProt accession no. | P02771  |
| Application: | Monoclonal Anti-α-Fetoprotein (AFP) antibody produced in mouse has been used in: • enzyme-linked immunosorbent assay (ELISA) • dot blot • immunocytochemistry • immunofluorescence • immunocytochemistry |
| Application: | Mouse Monoclonal Anti-α-Fetoprotein has been used for immunocytochemistry. The product can also be used for dot blot and indirect ELISA. |
| Biochem/physiol Actions: | Increased concentrations of α-fetoprotein (AFP) in serum are less common in patients with malignancies of the gastrointestinal tract and of other organ systems with massive hepatic metastases. Hence, AFP determination is useful for the diagnosis of certain germ line tumors that contain yolk sac structures (testicular and ovarian tumors), hepatocellular carcinoma (hepatoma), and some malignant tumors of the gastrointestinal tract. AFP serves as an important marker for certain genetic and embryonic defects. Routine screening of pregnancies with a risk of neural tube defects (spina bifida, anencephaly, and Down′s syndrome) may be facilitated by the monitoring of AFP levels. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | α-Fetoprotein (AFP) is a one of the main glycoprotein present in the fetal liver, gastrointestinal tract, and yolk sac. AFP levels in amniotic fluid have been used as biomarkers for anencephaly and spina bifida. |
| General description: | AFP is a plasma protein found highly expressed in the human fetus where it functions to block the transport of estradiol across the placenta. |
| General description: | Monoclonal Anti-α-Fetoprotein (AFP) (mouse IgG2a isotype) is derived from the C3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified human α-fetoprotein. |
| Immunogen: | human α-fetoprotein |
| Physical form: | The product is provided as ascites fluid with 0.1% sodium azide as a preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |