Anti-γ-Actin antibody, Mouse monoclonal
SIGMA/A8481 - clone 2-2.1.14.17, purified from hybridoma cell culture
Synonym: Anti-ACTG1
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human heart sections with 0.5 μg/mL Monoclonal Anti-γ-Actin, Clone: 2-2.1.14.17 (Cat. No. A8481) using Biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence Rat2 cells were fixed and permeabilized with methanol followed by methanol:aceton. Fixed cells were stained with 0.25 μg/mL Monoclonal Anti-γ-Actin Clone: 2-2.1.14.17 (Cat. No. A8481). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunofluorescence Total 3T3 cells extract was separated on SDS-PAGE and incubated with serial concentrations of Monoclonal Anti-GAMMA Actin (Cat. No. A8481). The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 0.5 μg/mL antibody 3. 0.25 μg/mL antibody 4. 0.125 μg/mL antibody
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 0.5 μg/mL Monoclonal Anti-γ-Actin Clone: 2-2.1.14.17 (Cat. No. A8481). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa 2. JURKAT 3. COS7 4. P19 5. PC-12 6. RAT2 7. CHO 8. MDBK 9. MDCK
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2-2.1.14.17, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~43 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, chicken, canine, bovine, human, hamster |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| indirect ELISA: suitable | |
| western blot: 0.25-0.5 μg/mL using total cell extract of 3T3 cells | |
| UniProt accession no. | P63261  |
| Application: | Anti-γ-Actin antibody, Mouse monoclonal has been used in : • immunofluorescence • enzyme linked immunosorbent assay (ELISA) • western blotting • immunohistochemistry |
| Application: | Mouse monoclonal Anti-γ-Actin antibody has been used for immunofluorescence assays. The antibody can also be used for ELISA, IHC and western blot assays. |
| Application: | Protein lysates from human Jurkat T-leukemic cells were subjected to western blot and probed using monoclonal mouse anti-actin (AC-40) to ensure equal loading. |
| Biochem/physiol Actions: | Monoclonal Anti-γ-Actin recognizes human, bovine, canine, mouse, hamster and chicken γ-actin. |
| Biochem/physiol Actions: | The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin is significantly involved in the microfilament system. It participates in muscle contraction and gene regulation. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Increased levels of γ-actin have been linked to dystrophin loss and subsequent cell signaling and gene expression defects. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | γ-Actin is mainly present in sarcolemma and to a lesser extent in reticular lattice of normal muscles. Increased levels of γ-actin have been linked to dystrophin loss and subsequent cell signaling and gene expression defects. |
| General description: | Monoclonal Anti-γ-Actin (mouse IgG1 isotype) is derived from the hybridoma 2-2.1.14.17 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. Actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms. Actin isoforms show more than 90% overall sequence homology, but only 50-60% homology in their 18 NH2-terminal residues. Actin constitutes for around 15% of the total protein. It exists in globular and filamentous forms. |
| Other Notes: | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/acti. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |