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Anti-Glutathione-S-Transferase (GST)–Agarose antibody produced in rabbit

SIGMA/A8580 - IgG fraction of antiserum, PBS suspension

Synonym: Anti-Glutathione-S-Transferase; Anti-GST

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-A8580-1ML 1 mL
$969.00
1/EA
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antibody form IgG fraction of antiserum
antibody product type primary antibodies
biological source rabbit
clone polyclonal
conjugate agarose conjugate
form PBS suspension
mol wt antigen 27.5 kDa
Quality Level 200 
shipped in wet ice
should not react with porcine, bovine, human, rabbit, rat
storage temp. 2-8°C
target post-translational modification unmodified
technique(s) ELISA: suitable
  immunoprecipitation (IP): suitable
Application: Immunoprecipitation was performed on NIH 3T3 cells transiently transfected with GST fusion proteins. IP was performed using agarose-linked rabbit anti-GST IgG with mixing for 2 hours at 4 degrees.
Application: Rabbit Anti-Glutathione-S-Transferase (GST)-Agarose antibody can be used for ELISA, immunoaffinity purification and immunoprecipitation assays.
Biochem/physiol Actions: Glutathione-S-transferases (GSTs) can catalyse the conjugation of glutathione (GSH) to various endogenous and exogenous electrophilic compounds. The π and μ classes of GSTs controls the mitogen-activated protein (MAP) kinase pathway. It can also serve as transport proteins.
Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description: Glutathione-S-transferases (GSTs) are dimeric proteins, which belongs to phase II detoxification enzymes family. The highly polymorphic human cytosolic GSTs are divided into six classes, such as, α, μ, ω, π, θ, and ζ.
General description: The antibody is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. Anti-GST may be used in various immunoassays to identify the expression of GST fusion proteins.
Immunogen: recombinant GST from Schistosoma japonicum expressed in E. coli.
Physical form: Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Preparation Note: Prepared by coupling cyanogen bromide-activated agarose at 7-8 mg antibody per mL resin volume.
Hazard Codes Xn
Risk Statements 21/22
Safety Statements 36/37
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. 2-8°C
UNSPSC 12352203

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