Antifoam SE-15
SIGMA/A8582 - aqueous emulsion for bacterial and mammalian systems
Synonym: silicone-based antifoam
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A8582-100G.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A8582-500G.
| application(s) | microbiology |
| concentration | 10% (active silicon) |
| density | 1.0 g/mL at 25 °C |
| form | emulsion (aqueous) |
| Quality Level | 200  |
| solubility | H2O: soluble (effective hot or cold.) |
| storage temp. | room temp |
| suitability | (Mammalian (suspension)) |
| bacteria (fermentation) | |
| technique(s) | cell culture | hybridoma: suitable |
| Application: | Antifoam SE-15 has been used: • in the preparation of ramified rolling circle amplification (RAM) amplification buffer. • to find its effects on cell viability, virus infectivity and PCR (polymerase chain reaction) performance. • as a constituent of phosphate-buffered saline (PBS) for the determination of total antioxidant status (TAOS) by 2,2′-azino-bis(3-ethylben • as a component of autoinduction expression culture. • as a component of cyclic adenosine monophosphate (cAMP) detection buffer. |
| Components: | Antifoam SE-15 is an aqueous emulsion containing 10% active silicon and non-ionic emulsifiers . |
| Features and Benefits: | • Controls foaming in microbial culture flasks or fermentations • Non-toxic to the culture medium • Can be pumped to fermenter on an as-needed basis |
| General description: | Antifoam SE-15 is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production. |
| Packaging: | 100, 500 g in glass bottle |
| Preparation Note: | Antifoam SE-15 can be pre-diluted with 3-10 parts of cool water to aid in dispersion. Pre-diluted suspensions should be used immediately. |
| Symbol |   GHS07,GHS08 |
| Signal word | Warning |
| Hazard statements | H319 - H373 |
| Precautionary statements | P260 - P305 + P351 + P338 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Density | 1.0 g/mL at 25 °C |
| Storage Temp. | room temp |
| UNSPSC | 12161901 |