Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody produced in mouse
SIGMA/A8592 - clone M2, purified immunoglobulin, buffered aqueous glycerol solution
Synonym: Monoclonal ANTI-FLAG® M2 antibody produced in mouse; Anti-ddddk; Anti-dykddddk
Product Type: Chemical
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | M2, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | peroxidase conjugate |
| form | buffered aqueous glycerol solution |
| immunogen sequence | DYKDDDDK |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | all |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: 1:20,000 |
| Application: | For simple, one-step detection by immunocytochemistry, ELISA, or Western blotting. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies may cause cross-reactivity. |
| Application: | Suggested dilution for immunocytochemistry and western blotting 1:100 to 1:1000 Suggested dilution for ELISA 1:20,000 Learn more product details in our FLAG® application portal. |
| General description: | The Monoclonal ANTI-FLAG M2-Peroxidase is a mouse IgG antibody covalently conjugated to horseradish peroxidase ( HRP). The antibody binds to FLAG fusion proteins and recognizes the FLAG epitope at N-terminal, Met-N-terminal, C-terminal, and internal FLAG peptides. |
| Legal Information: | ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Packaging: | 0.2, 1 mg in poly bottle |
| Physical form: | Solution in phosphate buffered saline containing 50% glycerol plus preservative and stabilizer |
| Preparation Note: | Dilute ANTI-FLAG M2-Peroxidase solution in Tris Buffered Saline (TBS): 0.05 M Tris, pH 7.4, with 0.15 M NaCl. |
| Symbol |   GHS07,GHS09 |
| Signal word | Warning |
| Hazard statements | H315 - H317 - H319 - H411 |
| Precautionary statements | P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 36/38-43-52/53 |
| Safety Statements | 26-36/37-61 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106514 |