Anti-Annexin V antibody, Mouse monoclonal
SIGMA/A8604 - clone AN5, purified from hybridoma cell culture
Synonym: Annexin V Antibody; Annexin V Antibody - Monoclonal Anti-Annexin V antibody produced in mouse
MDL Number: MFCD01864074
Product Type: Chemical
Immunoblotting
HeLa cell extract was separated by SDS-PAGE and probed with or without Monoclonal Anti-Annexin V, Clone AN5 (Cat. No. A8604) followed by Goat Anti-Mouse, Alkaline Phosphatase conjugate (Cat. No. A2179).
HeLa cell extract was separated by SDS-PAGE and probed with or without Monoclonal Anti-Annexin V, Clone AN5 (Cat. No. A8604) followed by Goat Anti-Mouse, Alkaline Phosphatase conjugate (Cat. No. A2179).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | AN5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~35 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.5-1 μg/mL using HeLa total cell extract | |
| UniProt accession no. | P08758  |
| Application: | Monoclonal Anti-Annexin V antibody produced in mouse has been used in: • immunocytochemistry • indirect immunofluorescence • immunohistochemistry • western blotting • enzyme linked immunosorbent assay (ELISA) |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Annexin V (mouse IgG1 isotype) is derived from the hybridoma AN5 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with purified human Annexin V. Annexin V belongs to a class of Ca2+-dependent binding proteins. the amino acid sequence of the annexin V protein consists of a core of four repeats of a highly conserved 70 amino acid residue motif and a unique N-terminal tail. Within each repeat, there is a 17 amino acid residue consensus sequence, which is postulated to form part of the Ca2+ and/or phospholipid binding site. |
| Immunogen: | purified human annexin V. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |