Avidin from egg white
SIGMA/A8706 - recombinant, expressed in corn, ≥12 units/mg protein (E1%/280 )
Synonym: AVID_CHICK
CAS Number: 1405-69-2
EC Number: 215-783-6
MDL Number: MFCD00130572
Product Type: Chemical
| assay | ≥80% protein basis (biuret) |
| form | powder |
| mol wt | glycoprotein 66 kDa |
| subunit 16 kDa | |
| Quality Level | 200  |
| recombinant | expressed in corn |
| specific activity | ≥12 units/mg protein (E |
| storage temp. | 2-8°C |
| technique(s) | ELISA: suitable |
| immunoblotting: suitable | |
| immunoelectrophoresis: suitable | |
| UniProt accession no. | P02701  |
| Application: | Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol. |
| Application: | Avidin forms an extremely strong complex with d-biotin (Kd ~ 10-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation. |
| General description: | Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties except for the molecular weight. Avidin from corn has low molecular weight than chicken egg-derived avidin. This might be due to less complex glycosylation composition in plants. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus. |
| Packaging: | 5 mg in glass bottle |
| Preparation Note: | Affinity purified |
| Unit Definition: | One unit will bind 1.0 μg of d-biotin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥80% protein basis (biuret) |
| activity | specific activity: ≥12 units/mg protein (E |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |