Anti-Amyloid Precursor Protein, C-Terminal antibody produced in rabbit
SIGMA/A8717 - IgG fraction of antiserum, buffered aqueous solution
Synonym: APP antibody; Anti-APP
MDL Number: MFCD02095828
Product Type: Chemical
Western Blotting JOURNAL CITATION: Alzheimer′s disease-linked mutations in presenilin-1 result in a drastic loss of activity in purified γ-secretase complexes. By: Cacquevel, M., Aeschbach, L., et al. in PLoS One, 2012. PubMed ID: 22529981 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0035133), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Characterization of a Drosophila Alzheimer′s disease model: pharmacological rescue of cognitive defects. By: Chakraborty, R., Vepuri, V., et al. in PLoS One, 2011. PubMed ID: 21673973 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0020799), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Silencing of amyloid precursor protein expression using a new engineered delta ribozyme. By: Ben Aissa, M., April, M. C., et al. in Int J Alzheimers Dis, 2012. PubMed ID: 22482079 Image collected and cropped by CiteAb from the following publication, (http://www.hindawi.com/journals/ijad/2012/947147/), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Rat cortex tissue stained with Rabbit Anti-Amyloid Precursor Protein, C-Terminal (Cat. No. A8717). From Dr. Shai Shoham, Research Laboratory, Sarah Herzog Memorial Hospital, Jerusalem, Israel
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Amyloid Precursor Protein, C-Terminal antibody produced in Rabbit (Cat. No. A8717) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Amyloid Precursor Protein (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:1,000.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human Alzheimer’s Disease (AD) brain sections with 1:1,000 Rabbit Anti-Amyloid Precursor Protein, C-Terminal (Cat. No. A8717) using biotin/ExtrAvidin®-Peroxidase.
Immunoblotting Rat brain cytosolic S1 fraction was separated on SDS-PAGE and probed with IgG Fraction AB APP, C-Terminal (RB) (BM# 3088) (Cat. No. A8717). The antibody was developed with Alk’ Phos’ APA Rabbit IgG (Cat. No. A9919) and NBT/BCIP substrate. Antibody dilution 1/8,000.
Immunoblotting Cell lysates of HEK-293T overexpressing human amyloid precursor protein (hAPP) (lane 1) and HEK-293T non-transfected (lane 2) were separated on SDS-PAGE, blotted with Anti-Amyloid Precursor Protein (C-terminal) (antibody dilution 1:1,000) (Cat. No. A8717), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Bands at 100 kDa and 120 kDa respectively, may represent differentially N- and O-glycosylated forms of human APP.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 95-100 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1000 using formic acid-treated sections of human Alzheimer′s disease (AD) brain |
| microarray: suitable | |
| western blot: 1:4,000 using rat brain extract | |
| UniProt accession no. | P05067  |
| Application: | Rabbit Anti-Amyloid Precursor Protein, C-Terminal antibody has been used for immunoprecipitation, immunohistochemical analysis, immunohistochemical staining and western blot applications. The product can also be used for IHC (1:1,000) and microarray studies. |
| Application: | SH-SY5Y cell lysates were analyzed by western blot using rabbit anti-Amyloid Precursor Protein, C-Terminal as the primary antibody at a 1:500 dilution. |
| Biochem/physiol Actions: | In transiently transfected cell lines, APP (amyloid precursor protein) regulates cell growth, motility, neurite outgrowth and cell survival. The intracellular C-terminus of APP acts as a transcriptional regulator and its amino acid domain serves as a regulator of its own intracellular sorting. Mutation of the gene leads to cerebral amyloid angiopathy. Accumulation of the cleavage products of APP, such as the β-amyloid peptide can cause Alzheimer′s disease. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Amyloid precursor proteins (APPs) are transmembrane glycoproteins that are found in a wide range of tissues. APP is expressed in the brain. It is located on human chromosome 21. APPs have 3 main isoforms, namely, APP695, APP751 and APP770 that are derived from alternative splicing events in cells. Accumulation of the cleavage products of APP, such as the β-amyloid peptide, can cause Alzheimer′s disease. |
| General description: | The immunogen sequence is identical to rat and mouse APP695 and in the APP isoforms APP751 and APP770. The antibody recognizes APP695, APP751 and APP770. |
| Immunogen: | Synthetic peptide corresponding to the C-terminal of human APP695 conjugated to KLH. |
| Physical form: | Supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative. |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |