Anti-Human IgG (whole molecule)−Peroxidase antibody produced in rabbit
SIGMA/A8792 - IgG fraction of antiserum, buffered aqueous solution
Synonym: HRP Rabbit Anti-Human IgG (whole molecule)
MDL Number: MFCD00162456
Product Type: Chemical
Western Blotting JOURNAL CITATION: Apolipoprotein J/Clusterin is a novel structural component of human erythrocytes and a biomarker of cellular stress and senescence. By: Antonelou, M. H., Kriebardis, A. G., et al. in PLoS One, 2011. PubMed ID: 21998749 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0026032), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Formalin-fixed, paraffin-embedded section of tonsil stained with 1:150 Anti-Human IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A8792).
| antibody form | IgG fraction of antiserum |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:40,000 |
| dot blot: 1:80,000 (chemiluminescent) | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150 |
| Application: | Anti-Human IgG (whole molecule)-Peroxidase antibody produced in rabbit has been used in: enzyme-linked immunosorbent assay (ELISA) western blotting microarray analysis |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Enzyme-linked immunosorbent assay (1 paper) Western Blotting (1 paper) |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. |
| General description: | Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. IgG is usually found as a monomer. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 percent of the total immunoglobulin consists of IgG. |
| Immunogen: | purified human IgG |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT |
| Preparation Note: | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| Specificity: | Rabbit Anti-Human IgG (whole molecule)-Peroxidase antibody binds to human IgG. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |