Aldolase from rabbit muscle
SIGMA/A8811 - ammonium sulfate suspension, 10-20 units/mg protein
Synonym: D-
CAS Number: 9024-52-6
MDL Number: MFCD00130453
Product Type: Chemical
| biological source | rabbit muscle |
| foreign activity | glyceraldehyde-3-phosphat |
| lactic dehydrogenase ≤0.03% | |
| phosphoglucose isomerase ≤0.6% | |
| pyruvate kinase ≤0.1% | |
| triosephosphate isomerase ≤0.05% | |
| form | ammonium sulfate suspension |
| Quality Level | 200  |
| specific activity | 10-20 units/mg protein |
| storage temp. | 2-8°C |
| Analysis Note: | Protein determined by biuret. |
| Application: | Aldolase from rabbit muscle has been used: • in standard 1-phosphofructokinase from rabbit muscle (RPFK-1) assay • as a standard in the characterization of metabolic enzymes from glaucomatous tissues • in fructose 2,6-bisphosphate assay of human cell lines |
| Application: | Aldolase is used to convert fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Aldolase, from rabbit muscle has been used for stereospecific deprotonation at DHAP C3 . |
| Biochem/physiol Actions: | Aldolase interaction with Wiskott-Aldrich syndrome protein (WASP) may modulate actin dynamics. It reverses the inhibition elicited by ascorbate on Muscle-type LDH (LDH-m4). |
| Biochem/physiol Actions: | Aldolase is involved in gluconeogenesis, the Calvin cycle and glycolysis. Aldolase, from rabbit muscle, is a class I aldolase which forms covalent Schiff base intermediates. The active site of aldolase is in the center of the α/β 8 barrel fold . |
| General description: | Aldolase exists as three isoforms in rabbit, which includes type A from muscle, type B from liver and brain associated type C. Aldolases correspond to a molecular weight of 158 kDa and exists as tetramer. |
| Packaging: | 100, 200, 1000 units in poly bottle |
| Packaging: | 5000 units in glass bottle |
| Physical form: | Crystalline suspension in 2.5 M (NH4)2SO4, 0.01 M Tris, pH 7.5, 0.001 M EDTA |
| Unit Definition: | One unit will convert 1.0 μmole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per min at pH 7.4 at 25 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 10-20 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 4.1.2.13 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |