Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit
SIGMA/A8919 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti Goat Hrp Sigma
MDL Number: MFCD00162340
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human tonsil tissue sections were stained with 1:50 Anti-Human IgG (whole molecule) antibody produced in Goat (Cat. No. I1011) followed by 1:200 Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A8919).
ELISA Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A8919) was serially diluted to detect Goat IgG by direct ELISA.
| antibody form | IgG fraction of antiserum |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:30,000 |
| dot blot: 1:2,000 | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 | |
| western blot (chemiluminescent): 1:160,000 |
| Application: | Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in rabbit has been used in immunoblotting and immunohistochemistry. |
| Biochem/physiol Actions: | Immunoglobulin mainly helps in immune defense. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reaction. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulin is a glycoprotein, with two heavy chain and two light chain connected by disulfide bond. Immunoglobulin g (IgG) is a major class of immunoglobulin. Goat IgG has two subclasses- IgG1 and IgG2. Rabbit anti-Goat IgG is then conjugated to peroxidase by protein cross linking with 0.2% glutaraldehyde. The conjugate is provided as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT as a preservative. |
| Immunogen: | Purified goat IgG |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT. |
| Preparation Note: | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |