Anti-Amyloid Precursor Protein, N-Terminal antibody produced in rabbit
SIGMA/A8967 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-APP
MDL Number: MFCD02095830
Product Type: Chemical
Immunoblotting Rat brain cytosolic S1 fraction was separated on SDS-PAGE and probed with IgG Fraction AB Amyloid Precursor Protein ) (Cat. No. A8967). The antibody was developed with Alk’ Phos’ APA Rabbit IgG (Cat. No. A9919) and NBT/BCIP substrate. Antibody dilution 1/2,000.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 95-100 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using formic acid-treated sections of human Alzheimer′s disease (AD) brain |
| microarray: suitable | |
| western blot: 1:1,000 using rat brain extract or supernatant of 293T cells secreting APP. | |
| UniProt accession no. | P05067  |
| Application: | Anti-Amyloid Precursor Protein, N-Terminal antibody produced in rabbit has been used in: • western blotting • immunostaining • immunofluorescence |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper) |
| Application: | SH-SY5Y cell lysates were analyzed by western blot using rabbit anti-Amyloid Precursor Protein, C-Terminal as the primary antibody at a 1:500 dilution. |
| Biochem/physiol Actions: | Amyloid precursor proteins (APPs) regulates cell growth, motility, neurite outgrowth and cell survival. The intracellular C-terminus of APP serves as a transcriptional regulator and as a receptor for kinesin-1-mediated axonal transport. Alzheimer′s disease is characterized by deposition of amyloid in the central nervous system, in neurite plaques and on cerebral vasculature. Mutations in the APP gene are linked with rare forms of autosomal dominant familial Alzheimer′s Disease (FAD). APPs undergo post-translational processing including N- and O-glycosylation, phosphorylation and sulfation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Amyloid precursor proteins (APPs) are transmembrane glycoproteins that are found in a wide range of tissues. APPs have 3 main isoforms, namely, APP695, APP751 and APP770 that are derived from alternative splicing events in cells. It is expressed at high levels in the brain. APP gene is mapped to human chromosome 21q11.2-q21. It is a 695 amino acid protein which possesses a large ectodomain and comparatively short intracellular region. |
| General description: | The immunogen sequence is identical to the APP isoforms APP751 and APP770 and is highly conserved (single amino acid substitution) in rat and mouse APP695. The antibody recognizes APP695, APP751 and APP770. |
| Immunogen: | synthetic peptide corresponding to the N-terminal of human APP695 (amino acids 46-60) conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |