Anti-Chicken IgY (IgG) (whole molecule)−Peroxidase antibody produced in rabbit
SIGMA/A9046 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti Chicken Igy
MDL Number: MFCD00162225
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human Tonsil tissue sections were stained with 1:50 Chicken Anti-Human IgG followed by 1:1,000 Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A9046).
ELISA Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A9046) was serially diluted to detect Chicken IgY (IgG) by direct ELISA.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | chicken |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:30,000 |
| dot blot: 1:160,000 (indirect chemiluminescence) | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 |
| Application: | Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody produced in rabbit has been used in indirect enzyme-linked immunosorbent assay (ELISA), dierct ELISA, dot blot and immunohistochemistry. |
| Application: | Proteins isolated from rabbit glomeruli by iron oxide magnetization were subjected to western blot analysis using HRP-conjugated rabbit anti-chicken IgY as the secondary antibody. |
| Application: | Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody has been used for immunoprecipitation and western blot (1:2,000) assays. The antibody can also be used for direct ELISA (1:30,000), dot blot (1:12,000-1:15,000) and IHC (1:1,000) applications. |
| Biochem/physiol Actions: | Egg yolk IgY immunoglobulin acquired from hen have been studied extensively. It is useful in immunological assays and does interact with the rheumatoid factor. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus anti-chicken antibodies are useful analytical tools for various research applications. Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody binds to chicken IgG. |
| Immunogen: | purified chicken IgG |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT |
| Preparation Note: | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |