Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat
SIGMA/A9169 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti Rabbit Antibody - Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat; Anti Rabbit Antibody Sigma
MDL Number: MFCD00162788
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of human tonsil stained with Anti-Human IgG (whole molecule) antibody produced in Rabbit (Cat. No. I8635) followed by 1:400 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A9169)
Western Blotting Whole extract of HeLa cells was separated on SDS-PAGE and probed with Anti-Actin, N-terminal antibody produced in Rabbit (Cat. No. A2103). The antibody was developed using 1:80000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A9169) and a chemiluminescent substrate. Lanes 1. 1:80,000 antibody 2. 1:160,000 antibody 3. Negative control: without first antibody
| antibody form | IgG fraction of antiserum |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| Quality Level | 300  |
| shipped in | dry ice |
| species reactivity | rabbit |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:50,000 using using 5 μg/ml of rabbit IgG for coating and OPD substrate |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 | |
| western blot (chemiluminescent): 1:80,000-1:160,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per well) |
| Application: | Anti-Rabbit IgG (whole molecule)–Peroxidase antibody has been used in western blotting. Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody can be used for immunoblot assays at 1:1,000. The antibody can also be used for ELISA (1:5,000) and IHC (1:400) applications. |
| Application: | Cell-associated protein samples from E. coli cultures were analyzed by western blot using HRP-conjugated goat anti-rabbit IgG as the secondary antibody. |
| Application: | Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody. |
| Biochem/physiol Actions: | Immunoglobulins are glycoprotein antibodies that modulate several immune responses. Immunoglobulin G participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. |
| Biochem/physiol Actions: | Specificity of the Peroxidase Conjugated Anti-Rabbit IgG antibodies is determined by immunoelectrophoresis (IEP) versus normal rabbit serum and rabbit IgG. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. IgG is a major class of immunoglobulin. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE. It has only one IgG variant with one interheavy disulfide bridge. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody binds all rabbit Igs. |
| Immunogen: | purified rabbit IgG |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT |
| Preparation Note: | For continuous use, store at 0-5EC. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Preparation Note: | Prepared by two-step glutaraldehyde method. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |