Avidin−Agarose from egg white
SIGMA/A9207 - aqueous glycerol suspension
Synonym: Avidin agarose
MDL Number: MFCD00130572
Product Type: Chemical
Avidin, a tetrameric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs.
| biological source | egg white |
| conjugate | agarose conjugate |
| form | aqueous glycerol suspension |
| matrix | cross-linked 6% beaded agarose |
| matrix activation | epichlorohydrin |
| matrix attachment | amino |
| matrix spacer | 15 atoms |
| Quality Level | 200  |
| storage temp. | −20°C |
| Application: | Avidin is used for the purification of biotin containing proteins, which can enhance cellular binding and internalization of the antibody (Ab)-functionalized capsules compared with control human immunoglobulin (IgG)-functionalized capsules, suggesting these capsules can specifically interact with cancer cells through antibody/antigen recognition. |
| Application: | Avidin-Agarose from egg white has been used: • in the precipitation of biotin tagged protein by pull down assay in neuroblastoma cell line (SH-SY5Y ) • in the purification of Lewis(x) sugar binding proteins from Schistosoma mansoni • in avidin bead assay for purification of labelled biotin • for capture of glycoproteins from murine cornea samples |
| Biochem/physiol Actions: | Avidin finds biotechnological applications due to its high-affinity ligand binding property. Avidin interaction with biotin through its carbohydrate chains is widely exploited in biotechnological applications. In cancer cells, avidin-biotin systems helps in the in vivo localization and drug targeting in tumor cells. |
| General description: | Avidin comprises of 0.05% of total egg white protein. It is a glycosylated protein. It corresponds to a molecular weight of 16.5 kDa and exists as tetramer, which can bind a minimum of three biotin molecules. |
| Other Notes: | One unit will bind 1.0 μg of d-biotin. |
| Physical form: | Suspension in 50% glycerol, 0.01 M sodium phosphate, pH 7.0, containing 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106514 |