Annexin V FITC Conjugate from human placenta
SIGMA/A9210
Synonym: Annexin V; Calphosbindin I; Lipocortin V; PAP-1
Product Type: Chemical
Fluorescence Staining of Apoptopic Cells Apoptotic cells exhibit an externalization of phosphatidylserine on the plasma membrane which is then available for binding to annexins conjugated with fluorescent labels, such as FITC. Necrotic cells will bind to Propidium iodide (PI) to the cellular DNA in cells where cell membrane has been totally comromised. The addition of Staurosporin induces apoptosis.
| extent of labeling | 1-2 mol FITC per mol annexin V |
| form | buffered aqueous solution |
| Quality Level | 300  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| UniProt accession no. | P08758  |
| Application: | For detection of apoptotic cells by flow cytometry or fluorescence microscopy. |
| Biochem/physiol Actions: | Annexins are ubiquitous homologous proteins that bind phospholipids in the presence of calcium. |
| Biochem/physiol Actions: | Annexins are ubiquitous homologous proteins that bind phospholipids in the presence of calcium. The cellular changes involved in the apoptotic process include loss of phospholipid asymmetry during the early stages. This phenomenon is universal and is not limited to stimulus or to mammalian cells, but also occurs in insect and plant cells. In living cells, phosphatidylserine is transported to the inside of the lipid bilayer by the Mg2+ATP dependent enzyme, aminophospholipid translocase. At the onset of apoptosis, phosphatidylserine becomes translocated to the external surface of the cell membrane. Since the movement of phosphatidylserine from the internal membrane surface to the external surface is an early indicator of apoptosis, annexin V and its conjugates that interact strongly and specifically with phosphatidylserine may be used to detect apoptosis. Annexin V conjugates can be used to detect apoptotic cells significantly earlier than DNA-based assays. Fluorescent dye labeled annexin V have applications in flow cytometry, fluorescence microscopy, and laser scanning cytometry. |
| Packaging: | Package size based on protein content |
| Physical form: | Solution in 50 mM Tris-HCl, pH 7.5, containing 100 mM NaCl. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106305 |