L-Amino Acid Oxidase from Crotalus adamanteus

SIGMA/A9253 - Type I (dried venom)

Synonym: L-AAO; L-Amino acid: oxygen oxidoreductase (deaminating)

CAS Number: 9000-89-9
EC Number: 232-564-0
MDL Number: MFCD00131454
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-A9253-100MG	100 mg	$320.00 1/EA	Add To Favorites
45-A9253-250MG	250 mg	$605.00 1/EA	Add To Favorites

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Properties
Descriptions
Safety Info.
Basic Data

biological source	Crotalus adamanteus
form	powder
mol wt	~130 kDa
Quality Level	200
solubility	H₂O: soluble 1.0 mg/mL, clear(lit.)
storage temp.	−20°C
type	Type I
	Type I (dried venom)

Application:	L-Amino Acid Oxidase from Crotalus adamanteus has been used in the quantification of rid protein in imine deaminase activity.
Application:	L-amino acid oxidase (LAAO) is used to convert L-amino acids to their corresponding α-keto acids. L-amino acid oxidase, from Sigma, has been used in leucine aminopeptidase (LAP) activity assays. The enzyme has been immobilized and used in an enzymatic flow-injection procedure with chemiluminescence detection for on-site determination of L-alanine.
Biochem/physiol Actions:	L-Amino acid oxidase (LAAO) from Crotalus adamanteus requires Mg²⁺ for its activation. The N-terminal region is essential for FAD binding. The H₂O₂ generated by LAAO based oxidation reactions lead to prevention of platelet aggregation and induces edema and apoptosis. LAAO activity is inhibited by ethylenediaminetetraacetic acid (EDTA) and phenylmethylsulfonyl fluoride (PMSF).
General description:	L-Amino acid oxidase (LAAO) is a flavoprotein with a molecular weight of 130 kDa. It consists of two different subunits of approximately 70 kDa. Each molecule of holoenzyme has two flavin adenine dinucleotide (FAD) molecules. LAAO is a glycoprotein containing about 2-5% carbohydrate, including sialic acid. optimum pH is approximately 7.5. It occurs in many snake venoms apart from microorganisms and animal tissue, especially in kidney. In the N-terminal region, it has a βαβ domain with glutamic acid residues. LAAO imparts yellow color to venom.
Preparation Note:	Dissolves in water at 1 mg/mL concentration to form a clear solution.

Symbol	GHS06
Signal word	Danger
Hazard statements	H300 + H310 + H330
Precautionary statements	P262 - P280 - P301 + P310 + P330 - P302 + P352 + P310 - P304 + P340 + P310
Hazard Codes	T+
Risk Statements	26/27/28
Safety Statements	36/37/39-45
RIDADR	UN 3462 6.1 / PGI
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	−20°C
Enzyme Commission (EC) Number	1.4.3.2 ( BRENDA \| IUBMB )
UNSPSC	12352204

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