Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat
SIGMA/A9309 - affinity isolated antibody, buffered aqueous solution
Synonym: HRP Goat Anti-Mouse IgG (Fc specific)
MDL Number: MFCD00162629
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded section of human appendix stained with Monoclonal Anti-Actin, α-Smooth Musclet (Cat. No. A2547) followed by 1:200 Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in goat (Cat. No. A9309).
Western Blotting Whole extract of HeLa cells were separated on SDS-PAGE and probed with 1:1,000 Anti-β-Actin antibody produced in Mouse. The antibody was developed using Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in Goat (Cat. No. A9309). Lanes 1. 1:40,000 antibody 2. 1:80,000 antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:40,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 | |
| western blot: 1:40,000-1:80,000 using total cell extract of HeLa cells |
| Application: | Anti-Mouse IgG (Fc specific)-Peroxidase antibody has been used: • in immunoblotting • in immunohistochemistry • in enzyme linked immunosorbent assay (ELISA) • in agar block precipitin titration • in western blotting • in assessment of in vitro poly(ADP-ribose)polymeras |
| Application: | Indirect ELISA was performed on sera from mice immunized to SV40-Tag using HRP-conjugated goat anti-mouse Fc specific IgG. Antibody was used at a 1:1000 dilution for 30 minutes at 37 degrees. |
| Application: | Specificity of a new antibody for PND in blood from immunized mice was tested by Elisa using biotynlated PND and streptavidin coated plates. HRP conjugated goat anti-mouse IgG (Fc specific) was used as secondary. |
| Biochem/physiol Actions: | IgG antibodies help in neutralizing the effects caused by viruses and toxins. |
| Biochem/physiol Actions: | IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection. Horseradish Peroxidase (HRP) is an enzyme that catalyzes the conversion of chromogenic substrates such as o-phenylenediamine (OPD), 4-chloro-1-naphthol 3,3’,5,5’-tetramethylbenz |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Goat polyclonal Mouse IgG (Fc specific)–Peroxidase antibody is determined against normal mouse serum, mouse IgG (whole molecule), the Fc fragment of mouse IgG and the Fab fragment of mouse IgG, the conjugate is specific for mouse IgG and shows no reaction with the Fab fragment of mouse IgG. The conjugate only shows reactivity with mouse IgG (whole molecule) and the Fc fragment of mouse IgG, when tested in ELISA. The conjugate shows no reaction with the Fab fragment of mouse IgG, human IgG, IgA, IgM, or rat IgG in ELISA. |
| General description: | Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. |
| Legal Information: | Ampliflu is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Antibody adsorbed with human IgG and rat serum proteins. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT. |
| Preparation Note: | Adsorbed to reduce background staining with human or rat samples. |
| Preparation Note: | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |