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L-Alanine 4-nitroanilide hydrochloride

SIGMA/A9325 - protease substrate

Synonym: Ala-4-nitroanilide; H-Ala-pNA

CAS Number: 31796-55-1
Empirical Formula (Hill Notation): C9H11N3O3 · HCl
Molecular Weight: 245.66
EC Number: 250-811-0
MDL Number: MFCD00039088
Linear Formula: C9H11N3O3 · HCl
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-A9325-100MG 100 mg
$59.20
1/EA
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45-A9325-500MG 500 mg
$163.00
1/EA
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45-A9325-1G 1 g
$240.00
1/EA
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45-A9325-5G 5 g
$907.00
1/EA
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assay ≥99% (TLC)
form powder
InChI 1S/C9H11N3O3.ClH/c1-6(10)9(13)11-7-2-4-8(5-3-7)12(14)15;/h2-6H,10H2,1H3,(H,11,13);1H/t6-;/m0./s1
InChI key YEXRLSXNWLNHQR-RGMNGODLSA-N
Quality Level 200 
SMILES string Cl[H].C[C@H](N)C(=O)Nc1ccc(cc1)[N+]([O-])=O
solubility water: 50 mg/mL, clear to very slightly hazy
storage temp. −20°C
Application: L-Alanine 4-nitroanilide hydrochloride has been used to determine the activity of aminopeptidase in the intestinal segments and small intestine (jejunum and ileum).
Biochem/physiol Actions: L-Alanine-4-nitroanilide is used as a substrate to determine alanine aminopeptidase (AAP) activity. It is mainly chosen as a substrate, due to its relative specificity, higher hydrolysis rate and better solubility.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥99% (TLC)
Storage Temp. −20°C
UNSPSC 12352204

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