L-Alanine 4-nitroanilide hydrochloride
SIGMA/A9325 - protease substrate
Synonym: Ala-4-nitroanilide; H-Ala-pNA
CAS Number: 31796-55-1
Empirical Formula (Hill Notation): C9H11N3O3 · HCl
Molecular Weight: 245.66
EC Number: 250-811-0
MDL Number: MFCD00039088
Linear Formula: C9H11N3O3 · HCl
Product Type: Chemical
| assay | ≥99% (TLC) |
| form | powder |
| InChI | 1S/C9H11N3O3.ClH/c1-6(10) |
| InChI key | YEXRLSXNWLNHQR-RGMNGODLSA |
| Quality Level | 200 ![]() |
| SMILES string | Cl[H].C[C@H](N)C(=O)Nc1cc |
| solubility | water: 50 mg/mL, clear to very slightly hazy |
| storage temp. | −20°C |
| Application: | L-Alanine 4-nitroanilide hydrochloride has been used to determine the activity of aminopeptidase in the intestinal segments and small intestine (jejunum and ileum). |
| Biochem/physiol Actions: | L-Alanine-4-nitroanilide is used as a substrate to determine alanine aminopeptidase (AAP) activity. It is mainly chosen as a substrate, due to its relative specificity, higher hydrolysis rate and better solubility. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% (TLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |

